Interaction of steroids with the nuclear envelope

Y. A. Lefebvre, J. T. Venkatraman, E. J. Golsteyn, G. M. Howell

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Three approaches have been taken to determine the molecular mechanism by which steroid hormones traversed the nuclear envelope on their way to the genome. The first approach involved the characterization of steroid binding to nuclear enevelope preparations. The authors have characterized androgen binding to nuclear envelopes isolated from the rat ventral prostate, the rat liver, and androgen-responsive and androgen-unresponsive cell lines of the Shionogi mouse mammary carcinoma and glucocorticoid binding to rat liver. Relatively high affinity binding sites for steroids have been identified on nuclear envelopes. Importantly, the number and specificity of the sites correlates with the responsiveness of the tissue to the steroid. In the second approach, the authors have undertaken to identify the steroid binding site directly. As the characteristics of the rat ventral prostate site resembled those of the nuclear androgen receptor, the authors have begun purifying that receptor and have found fast protein liquid chromatography to be very effective. By affinity labelling studies, the dexamethasone binding site on the rat liver nuclear envelope has been identified as a peptide of molecular weight of approximately 90 000. The third approach the authors have used is to idenfity androgen-dependent peptides in nuclear envelope preparations. In both the rat ventral prostate and an androgen-responsive cell line of the Shionogi mouse mammary carcinoma, the authors have identified abundant androgen-dependent peptides. The relationship of these peptides to the binding sites identified by the first two approaches and their role in steroid transport is being investigated.

Original languageEnglish (US)
Pages (from-to)594-600
Number of pages7
JournalBiochemistry and Cell Biology
Volume64
Issue number6
DOIs
StatePublished - Jan 1 1986

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Nuclear Envelope
Androgens
Rats
Steroids
Liver
Prostate
Peptides
Binding Sites
Steroid hormones
Cells
Breast Neoplasms
Cell Line
Liquid chromatography
Androgen Receptors
Cytoplasmic and Nuclear Receptors
Liquid Chromatography
Labeling
Glucocorticoids
Genes
Molecular Weight

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Lefebvre, Y. A., Venkatraman, J. T., Golsteyn, E. J., & Howell, G. M. (1986). Interaction of steroids with the nuclear envelope. Biochemistry and Cell Biology, 64(6), 594-600. https://doi.org/10.1139/o86-082

Interaction of steroids with the nuclear envelope. / Lefebvre, Y. A.; Venkatraman, J. T.; Golsteyn, E. J.; Howell, G. M.

In: Biochemistry and Cell Biology, Vol. 64, No. 6, 01.01.1986, p. 594-600.

Research output: Contribution to journalArticle

Lefebvre, YA, Venkatraman, JT, Golsteyn, EJ & Howell, GM 1986, 'Interaction of steroids with the nuclear envelope', Biochemistry and Cell Biology, vol. 64, no. 6, pp. 594-600. https://doi.org/10.1139/o86-082
Lefebvre YA, Venkatraman JT, Golsteyn EJ, Howell GM. Interaction of steroids with the nuclear envelope. Biochemistry and Cell Biology. 1986 Jan 1;64(6):594-600. https://doi.org/10.1139/o86-082
Lefebvre, Y. A. ; Venkatraman, J. T. ; Golsteyn, E. J. ; Howell, G. M. / Interaction of steroids with the nuclear envelope. In: Biochemistry and Cell Biology. 1986 ; Vol. 64, No. 6. pp. 594-600.
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