Interaction of estrogen receptors α and β with estrogen response elements

Margaret A. Loven, Jennifer R Wood, Ann M. Nardulli

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

To understand how estrogen-responsive genes are regulated, we compared the abilities of estrogen receptors (ERs) α and β to bind to and activate transcription through the consensus vitellogenin A2 ERE and the imperfect pS2, vitellogenin B1, and oxytocin (OT) EREs. Transient transfection experiments demonstrated that ERα and ERβ induced the highest levels of transcription with the A2 ERE, intermediate levels of transcription with the OT ERE, and low levels of transcription with the pS2 and B1 EREs. ERα and ERβ had higher affinities for the A2 ERE than for any of the three imperfect EREs but similar affinities for the pS2, B1, and OT EREs in gel mobility shift assays. ERα had a higher affinity and was a more potent activator of transcription than ERβ. Interestingly, protease sensitivity assays demonstrated that A2, pS2, B1, and OT EREs induced distinct changes in ERα and ERβ conformation thereby providing different functional surfaces for interaction with regulatory proteins involved in control of estrogen-responsive genes.

Original languageEnglish (US)
Pages (from-to)151-163
Number of pages13
JournalMolecular and Cellular Endocrinology
Volume181
Issue number1-2
DOIs
StatePublished - Jul 5 2001

Fingerprint

Response Elements
Estrogen Receptors
Estrogens
Transcription
Oxytocin
varespladib methyl
Vitellogenins
Assays
Genes
Electrophoretic Mobility Shift Assay
Transfection
Conformations
Peptide Hydrolases
Gels

Keywords

  • Conformation
  • Estrogen receptor α
  • Estrogen receptor β
  • Receptor-DNA interaction
  • Transcription

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

Cite this

Interaction of estrogen receptors α and β with estrogen response elements. / Loven, Margaret A.; Wood, Jennifer R; Nardulli, Ann M.

In: Molecular and Cellular Endocrinology, Vol. 181, No. 1-2, 05.07.2001, p. 151-163.

Research output: Contribution to journalArticle

@article{80857a64845449dfa818d482c88953d6,
title = "Interaction of estrogen receptors α and β with estrogen response elements",
abstract = "To understand how estrogen-responsive genes are regulated, we compared the abilities of estrogen receptors (ERs) α and β to bind to and activate transcription through the consensus vitellogenin A2 ERE and the imperfect pS2, vitellogenin B1, and oxytocin (OT) EREs. Transient transfection experiments demonstrated that ERα and ERβ induced the highest levels of transcription with the A2 ERE, intermediate levels of transcription with the OT ERE, and low levels of transcription with the pS2 and B1 EREs. ERα and ERβ had higher affinities for the A2 ERE than for any of the three imperfect EREs but similar affinities for the pS2, B1, and OT EREs in gel mobility shift assays. ERα had a higher affinity and was a more potent activator of transcription than ERβ. Interestingly, protease sensitivity assays demonstrated that A2, pS2, B1, and OT EREs induced distinct changes in ERα and ERβ conformation thereby providing different functional surfaces for interaction with regulatory proteins involved in control of estrogen-responsive genes.",
keywords = "Conformation, Estrogen receptor α, Estrogen receptor β, Receptor-DNA interaction, Transcription",
author = "Loven, {Margaret A.} and Wood, {Jennifer R} and Nardulli, {Ann M.}",
year = "2001",
month = "7",
day = "5",
doi = "10.1016/S0303-7207(01)00491-9",
language = "English (US)",
volume = "181",
pages = "151--163",
journal = "Molecular and Cellular Endocrinology",
issn = "0303-7207",
publisher = "Elsevier Ireland Ltd",
number = "1-2",

}

TY - JOUR

T1 - Interaction of estrogen receptors α and β with estrogen response elements

AU - Loven, Margaret A.

AU - Wood, Jennifer R

AU - Nardulli, Ann M.

PY - 2001/7/5

Y1 - 2001/7/5

N2 - To understand how estrogen-responsive genes are regulated, we compared the abilities of estrogen receptors (ERs) α and β to bind to and activate transcription through the consensus vitellogenin A2 ERE and the imperfect pS2, vitellogenin B1, and oxytocin (OT) EREs. Transient transfection experiments demonstrated that ERα and ERβ induced the highest levels of transcription with the A2 ERE, intermediate levels of transcription with the OT ERE, and low levels of transcription with the pS2 and B1 EREs. ERα and ERβ had higher affinities for the A2 ERE than for any of the three imperfect EREs but similar affinities for the pS2, B1, and OT EREs in gel mobility shift assays. ERα had a higher affinity and was a more potent activator of transcription than ERβ. Interestingly, protease sensitivity assays demonstrated that A2, pS2, B1, and OT EREs induced distinct changes in ERα and ERβ conformation thereby providing different functional surfaces for interaction with regulatory proteins involved in control of estrogen-responsive genes.

AB - To understand how estrogen-responsive genes are regulated, we compared the abilities of estrogen receptors (ERs) α and β to bind to and activate transcription through the consensus vitellogenin A2 ERE and the imperfect pS2, vitellogenin B1, and oxytocin (OT) EREs. Transient transfection experiments demonstrated that ERα and ERβ induced the highest levels of transcription with the A2 ERE, intermediate levels of transcription with the OT ERE, and low levels of transcription with the pS2 and B1 EREs. ERα and ERβ had higher affinities for the A2 ERE than for any of the three imperfect EREs but similar affinities for the pS2, B1, and OT EREs in gel mobility shift assays. ERα had a higher affinity and was a more potent activator of transcription than ERβ. Interestingly, protease sensitivity assays demonstrated that A2, pS2, B1, and OT EREs induced distinct changes in ERα and ERβ conformation thereby providing different functional surfaces for interaction with regulatory proteins involved in control of estrogen-responsive genes.

KW - Conformation

KW - Estrogen receptor α

KW - Estrogen receptor β

KW - Receptor-DNA interaction

KW - Transcription

UR - http://www.scopus.com/inward/record.url?scp=0035811531&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035811531&partnerID=8YFLogxK

U2 - 10.1016/S0303-7207(01)00491-9

DO - 10.1016/S0303-7207(01)00491-9

M3 - Article

VL - 181

SP - 151

EP - 163

JO - Molecular and Cellular Endocrinology

JF - Molecular and Cellular Endocrinology

SN - 0303-7207

IS - 1-2

ER -