Interaction of Bacillus species and Salmonella enterica serovar Typhimurium in immune or inflammatory signaling from swine intestinal epithelial cells

C. C. Aperce, Thomas E Burkey, B. KuKanich, B. A. Crozier-Dodson, S. S. Dritz, J. E. Minton

Research output: Contribution to journalArticle

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Abstract

Previous research evaluated a laboratory strain of Bacillus licheniformis (BL) in a model swine epithelium and found it exerted anti inflammatory effects on Salmonella enterica serovar Typhimurium (Sal)-induced secretion of IL-8. The current investigation evaluated the anti inflammatory actions of Bacillus bacteria available commercially as feed additives for the swine industry. Three isolates were obtained from the product, 2 Bacillus subtilis (BS1 and BS3) and 1 BL (BL2). Swine jejunal epithelial IPEC-J2 cells were seeded into wells on permeable membrane supports and allowed to form confluent monolayers. Treatments included apical pretreatment with BL, BS1, BL2, or BS3 for 17 h without Sal, and the same Bacillus treatments but with 108 cfu of Sal added in the final hour of Bacillus incubation. Two additional treatments included negative control wells receiving no bacteria (control) and positive control wells receiving only Sal (10 total treatments). After bacterial incubation, wells were washed and fresh medium containing gentamicin was added. Cells were incubated for an additional 5 h, after which apical and basolateral media were recovered for determination of IL-8 and bacitracin. In addition, inserts with epithelial cells that had received Sal were lysed and lysates were cultured to determine treatment effects on Sal invasion. Exposure to Sal alone provoked an increase in IL-8 secretion from IPEC-J2 cells compared with control wells (P < 0.001 for both the apical and basolateral directions). Pretreatment with each Bacillus isolate followed by challenge with Sal reduced Sal-induced IL-8 secretion in both the apical and basolateral compartments compared with wells receiving only Sal (P < 0.001; except for BS3 apical, P < 0.01). The residual presence of bacitracin could be detected only in BL2 and BL2+Sal. Fewer Sal colonies could be cultured from lysates of BL2+Sal than from the Sal, BS1+Sal, and BS3+Sal treatments (P < 0.001). Results indicate that B. subtilis and BL have the ability to intervene in secretion of the neutrophil chemoattractant IL-8 from swine intestinal epithelial cells. This effect on chemokine secretion by gastrointestinal epithelial cells in vitro could not be explained solely by reduced invasion of epithelial cells by Sal.

Original languageEnglish (US)
Pages (from-to)1649-1656
Number of pages8
JournalJournal of animal science
Volume88
Issue number5
DOIs
StatePublished - May 1 2010

Fingerprint

Salmonella enterica
Bacillus (bacteria)
Interleukin-8
Salmonella Typhimurium
Bacillus
interleukin-8
Bacillus licheniformis
epithelial cells
Swine
Epithelial Cells
swine
secretion
Bacitracin
bacitracin
Bacillus subtilis
Anti-Inflammatory Agents
Bacteria
pretreatment
pork industry
Chemotactic Factors

Keywords

  • Bacillus
  • Intestinal epithelium
  • Salmonella
  • Swine

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Genetics

Cite this

Interaction of Bacillus species and Salmonella enterica serovar Typhimurium in immune or inflammatory signaling from swine intestinal epithelial cells. / Aperce, C. C.; Burkey, Thomas E; KuKanich, B.; Crozier-Dodson, B. A.; Dritz, S. S.; Minton, J. E.

In: Journal of animal science, Vol. 88, No. 5, 01.05.2010, p. 1649-1656.

Research output: Contribution to journalArticle

Aperce, C. C. ; Burkey, Thomas E ; KuKanich, B. ; Crozier-Dodson, B. A. ; Dritz, S. S. ; Minton, J. E. / Interaction of Bacillus species and Salmonella enterica serovar Typhimurium in immune or inflammatory signaling from swine intestinal epithelial cells. In: Journal of animal science. 2010 ; Vol. 88, No. 5. pp. 1649-1656.
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