Integrating image-based phenomics and association analysis to dissect the genetic architecture of temporal salinity responses in rice

Malachy T. Campbell, Avi C. Knecht, Bettina Berger, Chris J. Brien, Dong Wang, Harkamal Walia

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Salinity affects a significant portion of arable land and is particularly detrimental for irrigated agriculture, which provides one-third of the global food supply. Rice (Oryza sativa), the most important food crop, is salt sensitive. The genetic resources for salt tolerance in rice germplasm exist but are underutilized due to the difficulty in capturing the dynamic nature of physiological responses to salt stress. The genetic basis of these physiological responses is predicted to be polygenic. In an effort to address this challenge, we generated temporal imaging data from 378 diverse rice genotypes across 14 d of 90 mM NaCl stress and developed a statistical model to assess the genetic architecture of dynamic salinity-induced growth responses in rice germplasm. A genomic region on chromosome 3 was strongly associated with the early growth response and was captured using visible range imaging. Fluorescence imaging identified four genomic regions linked to salinity-induced fluorescence responses. A region on chromosome 1 regulates both the fluorescence shift indicative of the longer term ionic stress and the early growth rate decline during salinity stress. We present, to our knowledge, a new approach to capture the dynamic plant responses to its environment and elucidate the genetic basis of these responses using a longitudinal genome-wide association model.

Original languageEnglish (US)
Pages (from-to)1476-1489
Number of pages14
JournalPlant physiology
Volume168
Issue number4
DOIs
StatePublished - Aug 1 2015

Fingerprint

Salinity
salinity
plant response
rice
image analysis
fluorescence
salt stress
germplasm
chromosomes
genomics
Growth
Salts
Fluorescence
food crops
Salt-Tolerance
arable soils
statistical models
salt tolerance
genetic resources
Chromosomes, Human, Pair 3

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

Integrating image-based phenomics and association analysis to dissect the genetic architecture of temporal salinity responses in rice. / Campbell, Malachy T.; Knecht, Avi C.; Berger, Bettina; Brien, Chris J.; Wang, Dong; Walia, Harkamal.

In: Plant physiology, Vol. 168, No. 4, 01.08.2015, p. 1476-1489.

Research output: Contribution to journalArticle

Campbell, Malachy T. ; Knecht, Avi C. ; Berger, Bettina ; Brien, Chris J. ; Wang, Dong ; Walia, Harkamal. / Integrating image-based phenomics and association analysis to dissect the genetic architecture of temporal salinity responses in rice. In: Plant physiology. 2015 ; Vol. 168, No. 4. pp. 1476-1489.
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