Integrating evolutionary and functional approaches to infer adaptation at specific loci

Jay F. Storz, Christopher W. Wheat

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

Inferences about adaptation at specific loci are often exclusively based on the static analysis of DNA sequence variation. Ideally, population-genetic evidence for positive selection serves as a stepping-off point for experimental studies to elucidate the functional significance of the putatively adaptive variation. We argue that inferences about adaptation at specific loci are best achieved by integrating the indirect, retrospective insights provided by population-genetic analyses with the more direct, mechanistic insights provided by functional experiments. Integrative studies of adaptive genetic variation may sometimes be motivated by experimental insights into molecular function, which then provide the impetus to perform population genetic tests to evaluate whether the functional variation is of adaptive significance. In other cases, studies may be initiated by genome scans of DNA variation to identify candidate loci for recent adaptation. Results of such analyses can then motivate experimental efforts to test whether the identified candidate loci do in fact contribute to functional variation in some fitness-related phenotype. Functional studies can provide corroborative evidence for positive selection at particular loci, and can potentially reveal specific molecular mechanisms of adaptation.

Original languageEnglish (US)
Pages (from-to)2489-2509
Number of pages21
JournalEvolution
Volume64
Issue number9
DOIs
StatePublished - Sep 1 2010

Fingerprint

Population Genetics
population genetics
loci
DNA Sequence Analysis
DNA
Genome
Phenotype
genetic variation
phenotype
fitness
experimental study
genome
testing
case studies
nucleotide sequences
experiment
test

Keywords

  • Adaptation
  • Fitness
  • Genomics
  • Mechanistic biology
  • Population genomics
  • Positive selection

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Agricultural and Biological Sciences(all)

Cite this

Integrating evolutionary and functional approaches to infer adaptation at specific loci. / Storz, Jay F.; Wheat, Christopher W.

In: Evolution, Vol. 64, No. 9, 01.09.2010, p. 2489-2509.

Research output: Contribution to journalArticle

@article{d26196ec1652495583b77917585d3ae5,
title = "Integrating evolutionary and functional approaches to infer adaptation at specific loci",
abstract = "Inferences about adaptation at specific loci are often exclusively based on the static analysis of DNA sequence variation. Ideally, population-genetic evidence for positive selection serves as a stepping-off point for experimental studies to elucidate the functional significance of the putatively adaptive variation. We argue that inferences about adaptation at specific loci are best achieved by integrating the indirect, retrospective insights provided by population-genetic analyses with the more direct, mechanistic insights provided by functional experiments. Integrative studies of adaptive genetic variation may sometimes be motivated by experimental insights into molecular function, which then provide the impetus to perform population genetic tests to evaluate whether the functional variation is of adaptive significance. In other cases, studies may be initiated by genome scans of DNA variation to identify candidate loci for recent adaptation. Results of such analyses can then motivate experimental efforts to test whether the identified candidate loci do in fact contribute to functional variation in some fitness-related phenotype. Functional studies can provide corroborative evidence for positive selection at particular loci, and can potentially reveal specific molecular mechanisms of adaptation.",
keywords = "Adaptation, Fitness, Genomics, Mechanistic biology, Population genomics, Positive selection",
author = "Storz, {Jay F.} and Wheat, {Christopher W.}",
year = "2010",
month = "9",
day = "1",
doi = "10.1111/j.1558-5646.2010.01044.x",
language = "English (US)",
volume = "64",
pages = "2489--2509",
journal = "Evolution; international journal of organic evolution",
issn = "0014-3820",
publisher = "Society for the Study of Evolution",
number = "9",

}

TY - JOUR

T1 - Integrating evolutionary and functional approaches to infer adaptation at specific loci

AU - Storz, Jay F.

AU - Wheat, Christopher W.

PY - 2010/9/1

Y1 - 2010/9/1

N2 - Inferences about adaptation at specific loci are often exclusively based on the static analysis of DNA sequence variation. Ideally, population-genetic evidence for positive selection serves as a stepping-off point for experimental studies to elucidate the functional significance of the putatively adaptive variation. We argue that inferences about adaptation at specific loci are best achieved by integrating the indirect, retrospective insights provided by population-genetic analyses with the more direct, mechanistic insights provided by functional experiments. Integrative studies of adaptive genetic variation may sometimes be motivated by experimental insights into molecular function, which then provide the impetus to perform population genetic tests to evaluate whether the functional variation is of adaptive significance. In other cases, studies may be initiated by genome scans of DNA variation to identify candidate loci for recent adaptation. Results of such analyses can then motivate experimental efforts to test whether the identified candidate loci do in fact contribute to functional variation in some fitness-related phenotype. Functional studies can provide corroborative evidence for positive selection at particular loci, and can potentially reveal specific molecular mechanisms of adaptation.

AB - Inferences about adaptation at specific loci are often exclusively based on the static analysis of DNA sequence variation. Ideally, population-genetic evidence for positive selection serves as a stepping-off point for experimental studies to elucidate the functional significance of the putatively adaptive variation. We argue that inferences about adaptation at specific loci are best achieved by integrating the indirect, retrospective insights provided by population-genetic analyses with the more direct, mechanistic insights provided by functional experiments. Integrative studies of adaptive genetic variation may sometimes be motivated by experimental insights into molecular function, which then provide the impetus to perform population genetic tests to evaluate whether the functional variation is of adaptive significance. In other cases, studies may be initiated by genome scans of DNA variation to identify candidate loci for recent adaptation. Results of such analyses can then motivate experimental efforts to test whether the identified candidate loci do in fact contribute to functional variation in some fitness-related phenotype. Functional studies can provide corroborative evidence for positive selection at particular loci, and can potentially reveal specific molecular mechanisms of adaptation.

KW - Adaptation

KW - Fitness

KW - Genomics

KW - Mechanistic biology

KW - Population genomics

KW - Positive selection

UR - http://www.scopus.com/inward/record.url?scp=77956407766&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77956407766&partnerID=8YFLogxK

U2 - 10.1111/j.1558-5646.2010.01044.x

DO - 10.1111/j.1558-5646.2010.01044.x

M3 - Article

C2 - 20500215

AN - SCOPUS:77956407766

VL - 64

SP - 2489

EP - 2509

JO - Evolution; international journal of organic evolution

JF - Evolution; international journal of organic evolution

SN - 0014-3820

IS - 9

ER -