Integrated generation of induced pluripotent stem cells in a low-cost device

Haishuang Lin, Qiang Li, Qian Du, Ou Wang, Zhanqi Wang, Leonard Akert, Mark Alan Carlson, Chi Zhang, Anuradha Subramanian, Chi Zhang, Matthew A Lunning, Ming Li, Yuguo Lei

Research output: Contribution to journalArticle

Abstract

Human induced pluripotent stem cells (iPSCs) have unlimited proliferation capability and potential to differentiate into all somatic cells. Their derivatives contain patients’ genetic information and can model many diseases. Additionally, derivatives of patient-specific iPSCs induce minimal immune rejection in vivo. With this unique combination of properties, iPSCs open the avenue to personalized medicine including personalized drug screening, toxicity test, cell therapy and tissue engineering. However, the further advance of iPSC-based personalized medicine is currently limited by the difficulty to generate iPSCs for large populations and at affordable cost. We here report a low-cost device to address this challenge. The device allows the entire bioprocess for generating high quality and quantity of iPSCs for one patient to be done automatically within a closed conical tube without cell passaging. Additionally, iPSCs can be further differentiated into somatic cells in the device. Thus, the device also allows integrated iPSCs generation, expansion and differentiation to produce any somatic cell types. This device can be made in large quantities at low cost for manufacturing iPSCs (and their derivatives in necessary) for large populations at affordable cost. It will significantly advance the iPSCs-based personalized medicine.

LanguageEnglish (US)
Pages23-36
Number of pages14
JournalBiomaterials
Volume189
DOIs
StatePublished - Jan 1 2019

Fingerprint

Induced Pluripotent Stem Cells
Stem cells
Costs and Cost Analysis
Equipment and Supplies
Costs
Precision Medicine
Medicine
Derivatives
Cell Engineering
Toxicity Tests
Preclinical Drug Evaluations
Tissue Engineering
Cell- and Tissue-Based Therapy
Drug-Related Side Effects and Adverse Reactions
Tissue engineering
Population
Toxicity
Screening

Keywords

  • Alginate hydrogel tubes
  • Human induced pluripotent stem cells
  • Personalized medicine
  • Reprogramming
  • Single-conical-tube device

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Integrated generation of induced pluripotent stem cells in a low-cost device. / Lin, Haishuang; Li, Qiang; Du, Qian; Wang, Ou; Wang, Zhanqi; Akert, Leonard; Carlson, Mark Alan; Zhang, Chi; Subramanian, Anuradha; Zhang, Chi; Lunning, Matthew A; Li, Ming; Lei, Yuguo.

In: Biomaterials, Vol. 189, 01.01.2019, p. 23-36.

Research output: Contribution to journalArticle

Lin, Haishuang ; Li, Qiang ; Du, Qian ; Wang, Ou ; Wang, Zhanqi ; Akert, Leonard ; Carlson, Mark Alan ; Zhang, Chi ; Subramanian, Anuradha ; Zhang, Chi ; Lunning, Matthew A ; Li, Ming ; Lei, Yuguo. / Integrated generation of induced pluripotent stem cells in a low-cost device. In: Biomaterials. 2019 ; Vol. 189. pp. 23-36.
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