Insulin modulation of bronchial epithelial cell fibronectin in vitro

Debra Romberger, P. Pladsen, L. Claassen, M. Yoshida, J. D. Beckmann, S. I. Rennard

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Fibronectin (Fn) is involved in the migration of epithelial cells in re- epithelialization of wounds. Epithelial cell-derived Fn is particularly potent as a chemotactic factor for bronchia/epithelial cells (BECs) in vitro. Thus modulation of airway epithelial cell Fn may be a key aspect of airway repair. Insulin is both an important growth factor and known chemotactic factor for cultured BECs. We postulated that insulin may modulate Fn production of cultured BECs. We examined this hypothesis utilizing bovine BECs in culture with serum-free media with and without insulin. BECs grown in media without insulin released more Fn into culture supernatants and contained more Fn in cell layers than cells grown with insulin. Labeling of cells with [35S]methionine demonstrated an increase in new protein production and Fn mRNA expression was increased. Increased Fn in BEC cultures without insulin was associated with an increase in active transforming growth factor-β (TGF-β) release as measured by a standard bioassay. Increased BEC Fn in cultures without insulin was partially inhibited by exposure of cultures to TGF-β antibody. Thus insulin appears to modulate BEC Fn production in vitro in part through a TGF-β-dependent mechanism. Insulin may be involved in airway repair mechanisms through modulation of epithelial cell Fn production.

Original languageEnglish (US)
Pages (from-to)L230-L238
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume268
Issue number2 12-2
StatePublished - Jan 1 1995

Fingerprint

fibronectins
Fibronectins
epithelial cells
insulin
Epithelial Cells
Insulin
transforming growth factors
Transforming Growth Factors
chemoattractants
Chemotactic Factors
cultured cells
In Vitro Techniques
cell culture
Cell Culture Techniques
Re-Epithelialization
Serum-Free Culture Media
cells
animal injuries
Biological Assay
Methionine

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

Cite this

Romberger, D., Pladsen, P., Claassen, L., Yoshida, M., Beckmann, J. D., & Rennard, S. I. (1995). Insulin modulation of bronchial epithelial cell fibronectin in vitro. American Journal of Physiology - Lung Cellular and Molecular Physiology, 268(2 12-2), L230-L238.

Insulin modulation of bronchial epithelial cell fibronectin in vitro. / Romberger, Debra; Pladsen, P.; Claassen, L.; Yoshida, M.; Beckmann, J. D.; Rennard, S. I.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 268, No. 2 12-2, 01.01.1995, p. L230-L238.

Research output: Contribution to journalArticle

Romberger, D, Pladsen, P, Claassen, L, Yoshida, M, Beckmann, JD & Rennard, SI 1995, 'Insulin modulation of bronchial epithelial cell fibronectin in vitro', American Journal of Physiology - Lung Cellular and Molecular Physiology, vol. 268, no. 2 12-2, pp. L230-L238.
Romberger, Debra ; Pladsen, P. ; Claassen, L. ; Yoshida, M. ; Beckmann, J. D. ; Rennard, S. I. / Insulin modulation of bronchial epithelial cell fibronectin in vitro. In: American Journal of Physiology - Lung Cellular and Molecular Physiology. 1995 ; Vol. 268, No. 2 12-2. pp. L230-L238.
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