Insulin-like growth factor binding protein-4 expression is dependent on the carbohydrate in the media in HT-29 cells

Mark R. Corkins, Jennifer McQuade, Beverly S. Schaffer, Richard G MacDonald

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

HT-29 are colonic carcinoma cells that follow a unique pattern of differentiation dependent on the medium's carbohydrate source. This study compared levels of insulin-like growth factor (IGF)-II and IGF binding protein (IGFBP)-4 when HT-29 cells were grown with standard glucose-containing medium versus galactose-containing (glucose-free) medium. Serum-free media conditioned for 24h were collected at low density, pre-confluence, confluence, and 48-h post-confluence. Ligand blotting of the conditioned galactose medium demonstrated low IGFBP-4 levels until the cells approached confluence, when the levels increased significantly. In standard medium, IGFBP-4 levels increased with increasing cell numbers except for a transient decrease at confluence. Radioimmunoassay showed little change in IGF-II concentrations, although HT-29 cells grown with galactose had lower IGF-II concentrations. HT-29 cells treated with retinoic acid had dose-dependent increases in IGFBP-4 and reduced IGF-II expression. These studies suggest that HT-29 cell differentiation correlates with an increase in IGFBP-4 levels.

Original languageEnglish (US)
Pages (from-to)184-192
Number of pages9
JournalGrowth Hormone and IGF Research
Volume12
Issue number3
DOIs
StatePublished - Jan 1 2002

Fingerprint

Insulin-Like Growth Factor Binding Protein 4
HT29 Cells
Insulin-Like Growth Factor II
Carbohydrates
Galactose
Glucose
Serum-Free Culture Media
Conditioned Culture Medium
Tretinoin
Radioimmunoassay
Cell Differentiation
Cell Count
Ligands
Carcinoma

Keywords

  • Differentiation
  • Entrocyte
  • HT-29
  • IGF-II
  • Insulin-like growth factor binding protein-4
  • Retinoic acid

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Insulin-like growth factor binding protein-4 expression is dependent on the carbohydrate in the media in HT-29 cells. / Corkins, Mark R.; McQuade, Jennifer; Schaffer, Beverly S.; MacDonald, Richard G.

In: Growth Hormone and IGF Research, Vol. 12, No. 3, 01.01.2002, p. 184-192.

Research output: Contribution to journalArticle

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