Insulin inhibits voltage-dependent calcium influx into rod photoreceptors

Jr Stella S.L., E. J. Bryson, Wallace B Thoreson

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Insulin inhibits the ERG b-wave and modulates L-type calcium currents (ICa) in various preparations. We therefore examined insulin's effects on ICa and depolarization-evoked [Ca2+]i increases in rod photoreceptors. Insulin inhibited ICa and caused a dose-dependent reduction in the depolarization-evoked Ca2+ influx with an EC50 of 2.1 nM. Tyrosine kinase inhibitors, lavendustin A (100nM) and genistein (10μM), prevented insulin from reducing the depolarization-evoked Ca2+ increase in rods. Their less active analogues, lavendustin B and daidzein, had similar effects. An insulin receptor-specific tyrosine kinase inhibitor, HNMPA-(AM)3 (50μM), prevented insulin (30 nM) from reducing the depolarization-evoked Ca2+ increase in rods. The results suggest that insulin inhibits Ca2+ influx through voltage-dependent ICa in rod photoreceptors via tyrosine kinase activity.

Original languageEnglish (US)
Pages (from-to)947-951
Number of pages5
JournalNeuroReport
Volume12
Issue number5
StatePublished - Apr 17 2001

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Retinal Rod Photoreceptor Cells
Insulin
Calcium
Protein-Tyrosine Kinases
Genistein

Keywords

  • Calcium channel
  • Calcium imaging
  • Fura-2
  • HNMPA-(AM)
  • Insulin
  • Lavendustin A and B
  • Retinal slice
  • Rod
  • Tyrosine kinase

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Insulin inhibits voltage-dependent calcium influx into rod photoreceptors. / Stella S.L., Jr; Bryson, E. J.; Thoreson, Wallace B.

In: NeuroReport, Vol. 12, No. 5, 17.04.2001, p. 947-951.

Research output: Contribution to journalArticle

Stella S.L., J, Bryson, EJ & Thoreson, WB 2001, 'Insulin inhibits voltage-dependent calcium influx into rod photoreceptors', NeuroReport, vol. 12, no. 5, pp. 947-951.
Stella S.L., Jr ; Bryson, E. J. ; Thoreson, Wallace B. / Insulin inhibits voltage-dependent calcium influx into rod photoreceptors. In: NeuroReport. 2001 ; Vol. 12, No. 5. pp. 947-951.
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