Insights regarding fungal phosphoproteomic analysis

Liliane F.C. Ribeiro, Cynthia L. Chelius, Steven D. Harris, Mark R. Marten

Research output: Contribution to journalReview article

2 Citations (Scopus)

Abstract

Protein phosphorylation is a major means of regulation for cellular processes, and is important in cell signaling, growth, and cell proliferation. To study phosphorylated proteins, high throughput phosphoproteomic technologies, such as reverse phase protein array, phospho-specific flow cytometry, and mass spectrometry (MS) based technologies, have been developed. Among them, mass spectrometry has become the primary tool employed for the identification of phosphoproteins and phosphosites in fungi, leading to an improved understanding of a number of signaling pathways. Using mass spectrometry techniques, researchers have discovered new kinase substrates, established connections between kinases and fungal pathogenicity, and studied the evolutionary lineage of kinases between different fungal species. Further, many specific phosphorylation sites recognized by individual kinases have been described. In this review, we will focus on recent discoveries made in yeast and filamentous fungi using phosphoproteomic analysis.

Original languageEnglish (US)
Pages (from-to)38-44
Number of pages7
JournalFungal Genetics and Biology
Volume104
DOIs
StatePublished - Jul 1 2017

Fingerprint

Phosphotransferases
Mass Spectrometry
Fungi
Phosphorylation
Technology
Protein Array Analysis
Phosphoproteins
Virulence
Flow Cytometry
Proteins
Yeasts
Research Personnel
Cell Proliferation
Growth

Keywords

  • Cellular regulation
  • Filamentous fungi
  • Mass spectrometry
  • Phosphorylation
  • Yeast

ASJC Scopus subject areas

  • Microbiology
  • Genetics

Cite this

Ribeiro, L. F. C., Chelius, C. L., Harris, S. D., & Marten, M. R. (2017). Insights regarding fungal phosphoproteomic analysis. Fungal Genetics and Biology, 104, 38-44. https://doi.org/10.1016/j.fgb.2017.03.003

Insights regarding fungal phosphoproteomic analysis. / Ribeiro, Liliane F.C.; Chelius, Cynthia L.; Harris, Steven D.; Marten, Mark R.

In: Fungal Genetics and Biology, Vol. 104, 01.07.2017, p. 38-44.

Research output: Contribution to journalReview article

Ribeiro, LFC, Chelius, CL, Harris, SD & Marten, MR 2017, 'Insights regarding fungal phosphoproteomic analysis', Fungal Genetics and Biology, vol. 104, pp. 38-44. https://doi.org/10.1016/j.fgb.2017.03.003
Ribeiro, Liliane F.C. ; Chelius, Cynthia L. ; Harris, Steven D. ; Marten, Mark R. / Insights regarding fungal phosphoproteomic analysis. In: Fungal Genetics and Biology. 2017 ; Vol. 104. pp. 38-44.
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