Inorganic arsenic-induced intramitochondrial granules in mouse urothelium

Shugo Suzuki, Lora L Arnold, David Muirhead, Lu Xiufen Lu, X. Chris Le, James A. Bjork, Kendall B. Wallace, Takamasa Ohnishi, Satoko Kakiuchi-Kiyota, Karen L. Pennington, Samuel Monroe Cohen

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Based on epidemiological data, chronic exposure to high levels of inorganic arsenic in the drinking water is carcinogenic to the urinary bladder of humans. Recently, models have been developed involving transplacental administration of inorganic arsenic and subsequent administration of another substance that produces a low incidence of urogenital neoplasms. Administration of arsenite or arsenate in the diet or drinking water to five-to eight-week-old mice or rats rapidly induces urothelial cytotoxicity and regenerative hyperplasia. In mice administered arsenite, we observed eosinophilic intracytoplasmic granules present in the urothelial cells. These granules were not present in urothelial cells of untreated mice or in treated or untreated rats. By transmission electron microscopy, the granules were located within the mitochondrial matrix, that is, mitochondrial inclusions. Arsenic, primarily as arsenite, was present in partially purified mitochondria containing these granules. Cells containing the granules were not usually associated with degenerative changes. Lack of these granules in rats suggests that they are not necessary for inorganic arsenic-induced urothelial cytotoxicity or hyperplasia. These granules have also been observed with exposures to other metals in other tissues and other species, suggesting that they represent a protective mechanism against metal-induced toxicity.

Original languageEnglish (US)
Pages (from-to)999-1005
Number of pages7
JournalToxicologic Pathology
Volume36
Issue number7
DOIs
StatePublished - Dec 1 2008

Fingerprint

Urothelium
Arsenic
Rats
Cytotoxicity
Drinking Water
Hyperplasia
Metals
Urogenital Neoplasms
Mitochondria
Nutrition
Transmission Electron Microscopy
Toxicity
Urinary Bladder
Cells
Tissue
Diet
Transmission electron microscopy
Incidence
arsenite

Keywords

  • Arsenite
  • Cytotoxicity
  • Hyperplasia
  • Mitochondrial granules
  • Urinary bladder

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Toxicology
  • Cell Biology

Cite this

Inorganic arsenic-induced intramitochondrial granules in mouse urothelium. / Suzuki, Shugo; Arnold, Lora L; Muirhead, David; Xiufen Lu, Lu; Le, X. Chris; Bjork, James A.; Wallace, Kendall B.; Ohnishi, Takamasa; Kakiuchi-Kiyota, Satoko; Pennington, Karen L.; Cohen, Samuel Monroe.

In: Toxicologic Pathology, Vol. 36, No. 7, 01.12.2008, p. 999-1005.

Research output: Contribution to journalArticle

Suzuki, S, Arnold, LL, Muirhead, D, Xiufen Lu, L, Le, XC, Bjork, JA, Wallace, KB, Ohnishi, T, Kakiuchi-Kiyota, S, Pennington, KL & Cohen, SM 2008, 'Inorganic arsenic-induced intramitochondrial granules in mouse urothelium', Toxicologic Pathology, vol. 36, no. 7, pp. 999-1005. https://doi.org/10.1177/0192623308327408
Suzuki, Shugo ; Arnold, Lora L ; Muirhead, David ; Xiufen Lu, Lu ; Le, X. Chris ; Bjork, James A. ; Wallace, Kendall B. ; Ohnishi, Takamasa ; Kakiuchi-Kiyota, Satoko ; Pennington, Karen L. ; Cohen, Samuel Monroe. / Inorganic arsenic-induced intramitochondrial granules in mouse urothelium. In: Toxicologic Pathology. 2008 ; Vol. 36, No. 7. pp. 999-1005.
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