Inhibitors of nitric oxide synthase attenuate human neutrophil chemotaxis in vitro

Sergei N. Belenky, Richard A. Robbins, Stephen I. Rennard, Gail L. Gossman, Kenneth J. Nelson, Israel Rubinstein

Research output: Contribution to journalArticle

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Abstract

Products released through the l-arginine/nitric oxide biosynthetic pathway regulate soluble guanyl cyclase activity, which in turn modulates polymorphonuclear leukocyte chemotaxis. We hypothesized that inhibitors of nitric oxide synthase attenuate polymorphonuclear leukocyte chemotaxis in vitro. To test this hypothesis, unstimulated polymorphonuclear leukocytes were pretreated with buffer or the nitric oxide synthase inhibitors NG-monomethyl-l-arginine (l-NMMA), NG-nitro-l-arginine methyl ester, and l-canavanine before being exposed to three structurally unrelated chemoattractants, N-formyl-methionyl-leucyl-phenylalanine, C5a des arginine, and leukotriene B4. Polymorphonuclear leukocyte chemotaxis was quantified with a modified blind-well chamber technique. We found that l-NMMA and l-canavanine but not NG-nitro-l-arginine significantly attenuated polymorphonuclear leukocyte chemotaxis (p < 0.05). l-Arginine but not d-arginine, the nitric oxide donor sodium nitroprusside, and 8-bromo-cyclic guanosine monophosphate restored polymorphonuclear leukocyte chemotaxis attenuated by l-NMMA. Chemotaxis of polymorphonuclear leukocytes primed with lipopolysaccharide (Escherichia coli 0127:B8) or phorbol-13-butyrate was also significantly attenuated by pretreatment with l-NMMA and l-canavanine. Consistent with these observations, intracellular concentrations of cyclic guanosine monophosphate in polymorphonuclear leukocytes was decreased by l-NMMA during exposure to N-formyl-methionyl-leucyl-phenylalanine. These data indicate that nitric oxide synthase inhibitors attenuate chemotaxis of unstimulated and primed polymorphonuclear leukocytes in vitro. We suggest that the larginine/nitric oxide biosynthetic pathway plays an important role in regulating polymorphonuclear leukocyte emigration in vivo. (J Lab Clin Med1993:122:388-94).

Original languageEnglish (US)
Pages (from-to)388-394
Number of pages7
JournalThe Journal of Laboratory and Clinical Medicine
Volume122
Issue number4
StatePublished - Oct 1993

Fingerprint

Chemotaxis
Nitric Oxide Synthase
Canavanine
Arginine
Neutrophils
Leukocyte Chemotaxis
N-Formylmethionine Leucyl-Phenylalanine
Cyclic GMP
des-Arginine Complement C5a
Nitric Oxide
Leukotriene B4
Nitric Oxide Donors
Butyrates
Guanylate Cyclase
Biosynthetic Pathways
Chemotactic Factors
Nitroprusside
Escherichia coli
Lipopolysaccharides
Buffers

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Belenky, S. N., Robbins, R. A., Rennard, S. I., Gossman, G. L., Nelson, K. J., & Rubinstein, I. (1993). Inhibitors of nitric oxide synthase attenuate human neutrophil chemotaxis in vitro. The Journal of Laboratory and Clinical Medicine, 122(4), 388-394.

Inhibitors of nitric oxide synthase attenuate human neutrophil chemotaxis in vitro. / Belenky, Sergei N.; Robbins, Richard A.; Rennard, Stephen I.; Gossman, Gail L.; Nelson, Kenneth J.; Rubinstein, Israel.

In: The Journal of Laboratory and Clinical Medicine, Vol. 122, No. 4, 10.1993, p. 388-394.

Research output: Contribution to journalArticle

Belenky, SN, Robbins, RA, Rennard, SI, Gossman, GL, Nelson, KJ & Rubinstein, I 1993, 'Inhibitors of nitric oxide synthase attenuate human neutrophil chemotaxis in vitro', The Journal of Laboratory and Clinical Medicine, vol. 122, no. 4, pp. 388-394.
Belenky SN, Robbins RA, Rennard SI, Gossman GL, Nelson KJ, Rubinstein I. Inhibitors of nitric oxide synthase attenuate human neutrophil chemotaxis in vitro. The Journal of Laboratory and Clinical Medicine. 1993 Oct;122(4):388-394.
Belenky, Sergei N. ; Robbins, Richard A. ; Rennard, Stephen I. ; Gossman, Gail L. ; Nelson, Kenneth J. ; Rubinstein, Israel. / Inhibitors of nitric oxide synthase attenuate human neutrophil chemotaxis in vitro. In: The Journal of Laboratory and Clinical Medicine. 1993 ; Vol. 122, No. 4. pp. 388-394.
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