Inhibition of protein kinase C epsilon causes ciliated bovine bronchial cell detachment

Rebecca E. Slager, Joseph Harold Sisson, Jaqueline A. Pavlik, Jennifer K. Johnson, Jeremy R. Nicolarsen, Thomas R. Jerrells, Todd A Wyatt

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

This study defines the in vitro phenomenon of ciliated bovine bronchial epithelial cell (BBEC) detachment from the basal epithelium and regulation of cilia motility mediated through protein kinase C epsilon (PKCε). The authors determined the time course of activation and downregulation of PKCε by the known PKC activator phorbol 12-myristate 13-acetate (PMA) and demonstrate that chemical inhibition of PKC by calphostin C or the novel PKC isoform inhibitor Ro 31-8220 induced striking detachment of ciliated BBECs from the basal cell monolayer within 1 hour, independent of apoptosis or necrotic cell death. The results of this study support a possible novel PKCε-mediated signaling pathway through which ciliated cell attachment is maintained.

Original languageEnglish (US)
Pages (from-to)349-362
Number of pages14
JournalExperimental Lung Research
Volume32
Issue number8
DOIs
StatePublished - Sep 1 2006

Fingerprint

Protein Kinase C-epsilon
Cilia
Cell death
Monolayers
Protein Isoforms
Acetates
Cell Death
Down-Regulation
Epithelium
Epithelial Cells
Chemical activation
Cells
Apoptosis

Keywords

  • Airway epithelium
  • Bronchial epithelial cell
  • Cell detachment
  • Cilia
  • Kinase
  • Protein kinase C epsilon

ASJC Scopus subject areas

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry

Cite this

Inhibition of protein kinase C epsilon causes ciliated bovine bronchial cell detachment. / Slager, Rebecca E.; Sisson, Joseph Harold; Pavlik, Jaqueline A.; Johnson, Jennifer K.; Nicolarsen, Jeremy R.; Jerrells, Thomas R.; Wyatt, Todd A.

In: Experimental Lung Research, Vol. 32, No. 8, 01.09.2006, p. 349-362.

Research output: Contribution to journalArticle

Slager, Rebecca E. ; Sisson, Joseph Harold ; Pavlik, Jaqueline A. ; Johnson, Jennifer K. ; Nicolarsen, Jeremy R. ; Jerrells, Thomas R. ; Wyatt, Todd A. / Inhibition of protein kinase C epsilon causes ciliated bovine bronchial cell detachment. In: Experimental Lung Research. 2006 ; Vol. 32, No. 8. pp. 349-362.
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