Inhibition of Listeria monocytogenes by bacteriocin-producing Pediococcus during the manufacture of fermented semidry sausage

Elaine D. Berry, Michael B. Liewen, Roger W. Mandigo, Robert W Hutkins

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

A bacteriocin-producing Pediococcus species inhibitory to Listeria monocytogenes was used to manufacture fermented semidry sausage. Separate 13.6 kg batches of a commercial summer sausage formulation were inoculated to contain an initial level of 106 cells/g of Listeria monocytogenes Scott A. In each of two independent studies, an ca. 2 log10 CFU/g reduction of L. monocytogenes occurred over the fermentation period, as compared to a less than 1 log10 CFU/g reduction in sausage fermented with a non-inhibitory Pediococcus strain. Inactivation of L. monocytogenes was also observed in one study where adequate acid production did not occur (pH>5.5), indicating that bacteriocin production occurred independently of carbohydrate fermentation. Following heating to an internal temperature of 64.4°C and storage up to 2 weeks, 9 of 90 sausages sampled were positive for Listeria. Recovery was intermittent and did not indicate that the bacteriocin was effective in eliminating L. monocytogenes that had survived the heating process.

Original languageEnglish (US)
Pages (from-to)194-197
Number of pages4
JournalJournal of Food Protection
Volume53
Issue number3
StatePublished - Mar 1 1990

Fingerprint

Pediococcus
Bacteriocins
bacteriocins
Listeria monocytogenes
manufacturing
Heating
Fermentation
summer sausage
fermentation
heat
Listeria
sausages
body temperature
inactivation
Carbohydrates
fermented meat
carbohydrates
Temperature
Acids
acids

ASJC Scopus subject areas

  • Food Science
  • Microbiology

Cite this

Inhibition of Listeria monocytogenes by bacteriocin-producing Pediococcus during the manufacture of fermented semidry sausage. / Berry, Elaine D.; Liewen, Michael B.; Mandigo, Roger W.; Hutkins, Robert W.

In: Journal of Food Protection, Vol. 53, No. 3, 01.03.1990, p. 194-197.

Research output: Contribution to journalArticle

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