Inhibition of glycolysis and respiration of sarcoma-180 cells by echitamine chloride

Saraswathi Viswanathan, N. Ramamoorthy, S. Subramaniam, V. Mathuram, P. Gunasekaran, S. Govindasamy

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Malignant tumors are known to exhibit high rates of glycolytic activity leading to high production of lactic acid. Hence, neoplastic cells have elevated activity of enzymes responsible for glycolysis. Echitamine chloride, an indole alkaloid extracted from the bark of Alstonia scholaris, has been reported to have a highly promising anticancer activity against fibrosarcoma in rats. In the present study, the effect of echitamine chloride on energy metabolism of S-180 cells is investigated to have a better understanding on the mode of action of echitamine chloride. The effect of echitamine chloride on the mitochondrial and cellular respiration of S-180 cells was studied. Also, the effects on glucose utilization, pyruvate utilization and lactate formation were studied on whole S-180 cells and S-180 cell-free homogenate. The levels of glycolytic enzymes such as hexokinase and lactate dehydrogenase were estimated in which particular emphasis has been laid on hexokinase which occurs both in cytosolic and particulate forms in neoplastic cells. Hence the differential effect of echitamine chloride on the levels of total, cytosolic and particulate hexokinase has been investigated. In conclusion, echitamine chloride affects both cellular and mitochondrial respiration, leading to reduction of the cellular energy pool and thereby resulting in the loss of viability of S-180 cells.

Original languageEnglish (US)
Pages (from-to)198-205
Number of pages8
JournalChemotherapy
Volume44
Issue number3
DOIs
StatePublished - Jan 1 1998

Fingerprint

Sarcoma 180
Glycolysis
Chlorides
Respiration
Hexokinase
Cell Respiration
Lactic Acid
Alstonia
Indole Alkaloids
Fibrosarcoma
Enzymes
echitamine
Pyruvic Acid
L-Lactate Dehydrogenase
Energy Metabolism
Glucose

Keywords

  • Echitamine chloride
  • Glucose
  • Hexokinase
  • Lactate
  • Lactate dehydrogenase
  • Oxygen uptake
  • Pyruvate
  • Sarcoma-180

ASJC Scopus subject areas

  • Oncology
  • Pharmacology
  • Drug Discovery
  • Pharmacology (medical)
  • Infectious Diseases

Cite this

Viswanathan, S., Ramamoorthy, N., Subramaniam, S., Mathuram, V., Gunasekaran, P., & Govindasamy, S. (1998). Inhibition of glycolysis and respiration of sarcoma-180 cells by echitamine chloride. Chemotherapy, 44(3), 198-205. https://doi.org/10.1159/000007115

Inhibition of glycolysis and respiration of sarcoma-180 cells by echitamine chloride. / Viswanathan, Saraswathi; Ramamoorthy, N.; Subramaniam, S.; Mathuram, V.; Gunasekaran, P.; Govindasamy, S.

In: Chemotherapy, Vol. 44, No. 3, 01.01.1998, p. 198-205.

Research output: Contribution to journalArticle

Viswanathan, S, Ramamoorthy, N, Subramaniam, S, Mathuram, V, Gunasekaran, P & Govindasamy, S 1998, 'Inhibition of glycolysis and respiration of sarcoma-180 cells by echitamine chloride', Chemotherapy, vol. 44, no. 3, pp. 198-205. https://doi.org/10.1159/000007115
Viswanathan S, Ramamoorthy N, Subramaniam S, Mathuram V, Gunasekaran P, Govindasamy S. Inhibition of glycolysis and respiration of sarcoma-180 cells by echitamine chloride. Chemotherapy. 1998 Jan 1;44(3):198-205. https://doi.org/10.1159/000007115
Viswanathan, Saraswathi ; Ramamoorthy, N. ; Subramaniam, S. ; Mathuram, V. ; Gunasekaran, P. ; Govindasamy, S. / Inhibition of glycolysis and respiration of sarcoma-180 cells by echitamine chloride. In: Chemotherapy. 1998 ; Vol. 44, No. 3. pp. 198-205.
@article{d688398f92a243d3a27dc5a73c4efff8,
title = "Inhibition of glycolysis and respiration of sarcoma-180 cells by echitamine chloride",
abstract = "Malignant tumors are known to exhibit high rates of glycolytic activity leading to high production of lactic acid. Hence, neoplastic cells have elevated activity of enzymes responsible for glycolysis. Echitamine chloride, an indole alkaloid extracted from the bark of Alstonia scholaris, has been reported to have a highly promising anticancer activity against fibrosarcoma in rats. In the present study, the effect of echitamine chloride on energy metabolism of S-180 cells is investigated to have a better understanding on the mode of action of echitamine chloride. The effect of echitamine chloride on the mitochondrial and cellular respiration of S-180 cells was studied. Also, the effects on glucose utilization, pyruvate utilization and lactate formation were studied on whole S-180 cells and S-180 cell-free homogenate. The levels of glycolytic enzymes such as hexokinase and lactate dehydrogenase were estimated in which particular emphasis has been laid on hexokinase which occurs both in cytosolic and particulate forms in neoplastic cells. Hence the differential effect of echitamine chloride on the levels of total, cytosolic and particulate hexokinase has been investigated. In conclusion, echitamine chloride affects both cellular and mitochondrial respiration, leading to reduction of the cellular energy pool and thereby resulting in the loss of viability of S-180 cells.",
keywords = "Echitamine chloride, Glucose, Hexokinase, Lactate, Lactate dehydrogenase, Oxygen uptake, Pyruvate, Sarcoma-180",
author = "Saraswathi Viswanathan and N. Ramamoorthy and S. Subramaniam and V. Mathuram and P. Gunasekaran and S. Govindasamy",
year = "1998",
month = "1",
day = "1",
doi = "10.1159/000007115",
language = "English (US)",
volume = "44",
pages = "198--205",
journal = "Chemotherapy",
issn = "0009-3157",
publisher = "S. Karger AG",
number = "3",

}

TY - JOUR

T1 - Inhibition of glycolysis and respiration of sarcoma-180 cells by echitamine chloride

AU - Viswanathan, Saraswathi

AU - Ramamoorthy, N.

AU - Subramaniam, S.

AU - Mathuram, V.

AU - Gunasekaran, P.

AU - Govindasamy, S.

PY - 1998/1/1

Y1 - 1998/1/1

N2 - Malignant tumors are known to exhibit high rates of glycolytic activity leading to high production of lactic acid. Hence, neoplastic cells have elevated activity of enzymes responsible for glycolysis. Echitamine chloride, an indole alkaloid extracted from the bark of Alstonia scholaris, has been reported to have a highly promising anticancer activity against fibrosarcoma in rats. In the present study, the effect of echitamine chloride on energy metabolism of S-180 cells is investigated to have a better understanding on the mode of action of echitamine chloride. The effect of echitamine chloride on the mitochondrial and cellular respiration of S-180 cells was studied. Also, the effects on glucose utilization, pyruvate utilization and lactate formation were studied on whole S-180 cells and S-180 cell-free homogenate. The levels of glycolytic enzymes such as hexokinase and lactate dehydrogenase were estimated in which particular emphasis has been laid on hexokinase which occurs both in cytosolic and particulate forms in neoplastic cells. Hence the differential effect of echitamine chloride on the levels of total, cytosolic and particulate hexokinase has been investigated. In conclusion, echitamine chloride affects both cellular and mitochondrial respiration, leading to reduction of the cellular energy pool and thereby resulting in the loss of viability of S-180 cells.

AB - Malignant tumors are known to exhibit high rates of glycolytic activity leading to high production of lactic acid. Hence, neoplastic cells have elevated activity of enzymes responsible for glycolysis. Echitamine chloride, an indole alkaloid extracted from the bark of Alstonia scholaris, has been reported to have a highly promising anticancer activity against fibrosarcoma in rats. In the present study, the effect of echitamine chloride on energy metabolism of S-180 cells is investigated to have a better understanding on the mode of action of echitamine chloride. The effect of echitamine chloride on the mitochondrial and cellular respiration of S-180 cells was studied. Also, the effects on glucose utilization, pyruvate utilization and lactate formation were studied on whole S-180 cells and S-180 cell-free homogenate. The levels of glycolytic enzymes such as hexokinase and lactate dehydrogenase were estimated in which particular emphasis has been laid on hexokinase which occurs both in cytosolic and particulate forms in neoplastic cells. Hence the differential effect of echitamine chloride on the levels of total, cytosolic and particulate hexokinase has been investigated. In conclusion, echitamine chloride affects both cellular and mitochondrial respiration, leading to reduction of the cellular energy pool and thereby resulting in the loss of viability of S-180 cells.

KW - Echitamine chloride

KW - Glucose

KW - Hexokinase

KW - Lactate

KW - Lactate dehydrogenase

KW - Oxygen uptake

KW - Pyruvate

KW - Sarcoma-180

UR - http://www.scopus.com/inward/record.url?scp=0031947447&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031947447&partnerID=8YFLogxK

U2 - 10.1159/000007115

DO - 10.1159/000007115

M3 - Article

VL - 44

SP - 198

EP - 205

JO - Chemotherapy

JF - Chemotherapy

SN - 0009-3157

IS - 3

ER -