Inhibition of carotid artery neointimal formation with intravenous microbubbles

Thomas Richard Porter, William L. Hiser, David Kricsfeld, Ubeydullah Deligonul, Feng Xie, Patrick Iversen, Stanley J Radio

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Because therapeutic gene products such as synthetic antisense oligodeoxynucleotides (ODN) bind to albumin-coated microbubbles, we sought to determine whether IV perfluorocarbon-exposed sonicated dextrose albumin (PESDA) microbubbles could target their delivery to the carotid artery following balloon injury. In 5 pigs, the concentration of ODN taken up within the carotid vascular wall was found to be significantly increased when the IV antisense (ODN) was administered bound to PESDA (ODN-PESDA), and while transcutaneous low-frequency (20 kHz) ultrasound was applied over the carotid artery. Based on these results, a chronic model was then developed, in which 21 pigs received either IV ODN-PESDA, ODN alone, or control, following carotid balloon injury. At 30 days following balloon injury, percent area stenosis was only 8 ± 2% in the ODN-PESDA groups compared to 19 ± 8% and 28 ± 3% in the other groups (p < 0.01). IV PESDA may be a method of noninvasively targeting the delivery of therapeutic genes. (E-mail: trporter@unmc.edu)

Original languageEnglish (US)
Pages (from-to)259-265
Number of pages7
JournalUltrasound in Medicine and Biology
Volume27
Issue number2
DOIs
StatePublished - Apr 28 2001

Fingerprint

Microbubbles
Oligodeoxyribonucleotides
Fluorocarbons
arteries
albumins
Carotid Arteries
Albumins
Glucose
balloons
swine
genes
delivery
Wounds and Injuries
Swine
Genes
Blood Vessels
Pathologic Constriction
low frequencies
Therapeutics
products

Keywords

  • Antisense oligonucleotides
  • Drug delivery
  • Microbubbles
  • Ultrasound

ASJC Scopus subject areas

  • Biophysics
  • Radiological and Ultrasound Technology
  • Acoustics and Ultrasonics

Cite this

Inhibition of carotid artery neointimal formation with intravenous microbubbles. / Porter, Thomas Richard; Hiser, William L.; Kricsfeld, David; Deligonul, Ubeydullah; Xie, Feng; Iversen, Patrick; Radio, Stanley J.

In: Ultrasound in Medicine and Biology, Vol. 27, No. 2, 28.04.2001, p. 259-265.

Research output: Contribution to journalArticle

Porter, Thomas Richard ; Hiser, William L. ; Kricsfeld, David ; Deligonul, Ubeydullah ; Xie, Feng ; Iversen, Patrick ; Radio, Stanley J. / Inhibition of carotid artery neointimal formation with intravenous microbubbles. In: Ultrasound in Medicine and Biology. 2001 ; Vol. 27, No. 2. pp. 259-265.
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