Inhibition of apoptosis in human tumour cells by okadaic acid

Qizhong Song, Glenn D. Baxter, Eva M. Kovacs, Duygu Findik, Martin F. Lavin

Research output: Contribution to journalArticle

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Abstract

Gamma‐radiation, tetrandrine, bistratene A, and cisplatin were all found to induce pronounced morphological changes characteristic of apoptosis and extensive DNA fragmentation in the human BM13674 cell line 8 h after treatment. Apoptosis induced in BM13674 cells by these diverse agents was markedly inhibited by 1 μM okadaic acid, a tumour promoter that inhibits protein phosphatases 1 and 2A. This compound also inhibited the appearance of apoptosis in fresh human leukaemia cells that had been exposed to gamma‐radiation. The inhibition of apoptosis was confirmed using fluorescence microscopy and DNA gel electrophoresis. Dephosphorylation of a limited number of proteins was shown to be associated with apoptosis and okadaic acid prevented these dephosphorylations. Previous studies on the BM13674 cell line showed that an inhibitor of protein synthesis failed to prevent apoptosis in these cells. The present data provides further support that posttranslational modification of proteins, in particular, phosphorylation/dephosphorylation status, plays an important role in inhibition/activation of programmed cell death in different human cells after exposure to several cytotoxic agents. © 1992 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)550-556
Number of pages7
JournalJournal of Cellular Physiology
Volume153
Issue number3
DOIs
StatePublished - Dec 1992

Fingerprint

Okadaic Acid
Tumors
Cells
Apoptosis
Neoplasms
tetrandrine
Protein Phosphatase 1
Protein Phosphatase 2
Cell Line
Phosphorylation
Protein Synthesis Inhibitors
Fluorescence microscopy
DNA
Cytotoxins
DNA Fragmentation
Cell death
Post Translational Protein Processing
Electrophoresis
Fluorescence Microscopy
Carcinogens

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Song, Q., Baxter, G. D., Kovacs, E. M., Findik, D., & Lavin, M. F. (1992). Inhibition of apoptosis in human tumour cells by okadaic acid. Journal of Cellular Physiology, 153(3), 550-556. https://doi.org/10.1002/jcp.1041530316

Inhibition of apoptosis in human tumour cells by okadaic acid. / Song, Qizhong; Baxter, Glenn D.; Kovacs, Eva M.; Findik, Duygu; Lavin, Martin F.

In: Journal of Cellular Physiology, Vol. 153, No. 3, 12.1992, p. 550-556.

Research output: Contribution to journalArticle

Song, Q, Baxter, GD, Kovacs, EM, Findik, D & Lavin, MF 1992, 'Inhibition of apoptosis in human tumour cells by okadaic acid', Journal of Cellular Physiology, vol. 153, no. 3, pp. 550-556. https://doi.org/10.1002/jcp.1041530316
Song, Qizhong ; Baxter, Glenn D. ; Kovacs, Eva M. ; Findik, Duygu ; Lavin, Martin F. / Inhibition of apoptosis in human tumour cells by okadaic acid. In: Journal of Cellular Physiology. 1992 ; Vol. 153, No. 3. pp. 550-556.
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