Inhibition of antigen-specific IgE production by antigen coupled to membrane IgE peptide

Swey Shen Chen, Robert Schmaltz, Yan Yu Wang, Qing Xian Kong, Thomas M Petro, Qing Li, Tse Wen Chang

Research output: Contribution to journalArticle

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Abstract

The membrane IgE peptide (MEP) encompassing 20 amino acids proximal to the C terminus of membrane IgE molecules, and secretory IgE peptides (SEP), spanning CHε 1 to 4 domain were synthesized according to IgE genomic and cDNA sequences. Inhibition of anti-KLH and anti-BGG IgE, but not IgG responses was observed in mice treated with MEP-protein but not SEP-protein conjugates in complete/incomplete Freund's adjuvant. Only IgE responses directed toward proteins to which MEP was conjugated, were inhibited, while IgE responses to a concomitantly injected, unrelated antigen were not. Inhibition of antigen-specific IgE was also not correlated with levels of anti-MEP or anti-IgE antibodies; moreover, levels of total IgE remained comparable among mice treated with MEP-protein conjugates, native or glutaraldehyde-modified protein carriers. This observation may have significant import on future design of IgE immunotherapy. Treatment of MEP conjugated allergens prevents formation of IgE-anti-IgE complexes because the MEP sequence is absent from the secretory IgE.

Original languageEnglish (US)
Pages (from-to)495-505
Number of pages11
JournalImmunological Investigations
Volume25
Issue number5-6
DOIs
StatePublished - Jan 1 1996

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Immunoglobulin E
Antigens
Peptides
Membranes
Proteins
Glutaral
Immunotherapy
Allergens
Carrier Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Inhibition of antigen-specific IgE production by antigen coupled to membrane IgE peptide. / Chen, Swey Shen; Schmaltz, Robert; Wang, Yan Yu; Kong, Qing Xian; Petro, Thomas M; Li, Qing; Chang, Tse Wen.

In: Immunological Investigations, Vol. 25, No. 5-6, 01.01.1996, p. 495-505.

Research output: Contribution to journalArticle

Chen, Swey Shen ; Schmaltz, Robert ; Wang, Yan Yu ; Kong, Qing Xian ; Petro, Thomas M ; Li, Qing ; Chang, Tse Wen. / Inhibition of antigen-specific IgE production by antigen coupled to membrane IgE peptide. In: Immunological Investigations. 1996 ; Vol. 25, No. 5-6. pp. 495-505.
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