Inhibiting Hexamer Disassembly of Human UDP-Glucose Dehydrogenase by Photoactivated Amino Acid Cross-Linking

George Grady, Ashley Thelen, Jaleen Albers, Tong Ju, Jiantao Guo, Joseph J. Barycki, Melanie A. Simpson

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The enzyme UDP-glucose dehydrogenase (UGDH) catalyzes the reaction of UDP-glucose to UDP-glucuronate through two successive NAD+-dependent oxidation steps. Human UGDH apoprotein is purified as a mixture of dimeric and hexameric species. Addition of substrate and cofactor stabilizes the oligomeric state to primarily the hexameric form. To determine if the dynamic conformations of hUGDH are required for catalytic activity, we used site-specific unnatural amino acid incorporation to facilitate cross-linking of monomeric subunits into predominantly obligate oligomeric species. Optimal cross-linking was achieved by encoding p-benzoyl-l-phenylalanine at position 458, normally a glutamine located within the dimer-dimer interface, and exposing the enzyme to long wavelength ultraviolet (UV) radiation in the presence of substrate and cofactor. Hexameric complexes were purified by gel filtration chromatography and found to contain significant fractions of dimer and trimer (approximately 50%) along with another 10% higher-molecular mass species. The activity of the cross-linked enzyme was reduced by almost 60% relative to that of the un-cross-linked UGDH mutant, and UV exposure had no effect on the activity of the wild-type enzyme. These results support a model for catalysis in which the ability to dissociate the dimer-dimer interface is as important for maximal enzyme function as has been previously shown for the formation of the hexamer.

Original languageEnglish (US)
Pages (from-to)3157-3164
Number of pages8
JournalBiochemistry
Volume55
Issue number22
DOIs
StatePublished - Jun 7 2016

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Uridine Diphosphate Glucose Dehydrogenase
Dimers
Amino Acids
Enzymes
Uridine Diphosphate Glucose
Glucuronic Acid
Apoproteins
Uridine Diphosphate
Molecular mass
Substrates
Chromatography
Glutamine
Catalysis
Phenylalanine
Catalyst supports
Ultraviolet radiation
NAD
Gel Chromatography
Conformations
Catalyst activity

ASJC Scopus subject areas

  • Biochemistry

Cite this

Inhibiting Hexamer Disassembly of Human UDP-Glucose Dehydrogenase by Photoactivated Amino Acid Cross-Linking. / Grady, George; Thelen, Ashley; Albers, Jaleen; Ju, Tong; Guo, Jiantao; Barycki, Joseph J.; Simpson, Melanie A.

In: Biochemistry, Vol. 55, No. 22, 07.06.2016, p. 3157-3164.

Research output: Contribution to journalArticle

Grady, George ; Thelen, Ashley ; Albers, Jaleen ; Ju, Tong ; Guo, Jiantao ; Barycki, Joseph J. ; Simpson, Melanie A. / Inhibiting Hexamer Disassembly of Human UDP-Glucose Dehydrogenase by Photoactivated Amino Acid Cross-Linking. In: Biochemistry. 2016 ; Vol. 55, No. 22. pp. 3157-3164.
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