Inheritance and expression of chimeric genes in the progeny of transgenic maize plants

M. E. Fromm, F. Morrish, C. Armstrong, R. Williams, J. Thomas, T. M. Klein

Research output: Contribution to journalArticle

Abstract

We obtained transgenic maize plants by using high-velocity microprojectiles to transfer genes into embryogenic cells. Two selectable genes were used to confer resistance to either chlorsulfuron or phosphinothricin, and genes encoding either E. coli β-glucuronidase or firefly luciferase were used as markers to provide convenient assays for transformation. When regenerated without selection, only two of the eight transformed embryogenic calli obtained produced transgenic maize plants. With selection, transgenic plants were obtained from three of the other eight calli. One of the two initial lines produced 15 fertile transgenic plants. The progeny of these plants contained and expressed the foreign genes. Luciferase expression could be visualized, in the presence of added luciferin, by overlaying leaf sections with color film.

Original languageEnglish (US)
Pages (from-to)833-839
Number of pages7
JournalBio/Technology
Volume8
Issue number9
DOIs
StatePublished - 1990
Externally publishedYes

Fingerprint

Genetically Modified Plants
Zea mays
Genes
Color films
Gene transfer
Firefly Luciferases
Gene Expression
Gene encoding
Glucuronidase
Bony Callus
Luciferases
Escherichia coli
Assays
Color
phosphinothricin
chlorsulfuron

ASJC Scopus subject areas

  • Biotechnology

Cite this

Fromm, M. E., Morrish, F., Armstrong, C., Williams, R., Thomas, J., & Klein, T. M. (1990). Inheritance and expression of chimeric genes in the progeny of transgenic maize plants. Bio/Technology, 8(9), 833-839. https://doi.org/10.1038/nbt0990-833

Inheritance and expression of chimeric genes in the progeny of transgenic maize plants. / Fromm, M. E.; Morrish, F.; Armstrong, C.; Williams, R.; Thomas, J.; Klein, T. M.

In: Bio/Technology, Vol. 8, No. 9, 1990, p. 833-839.

Research output: Contribution to journalArticle

Fromm, ME, Morrish, F, Armstrong, C, Williams, R, Thomas, J & Klein, TM 1990, 'Inheritance and expression of chimeric genes in the progeny of transgenic maize plants', Bio/Technology, vol. 8, no. 9, pp. 833-839. https://doi.org/10.1038/nbt0990-833
Fromm, M. E. ; Morrish, F. ; Armstrong, C. ; Williams, R. ; Thomas, J. ; Klein, T. M. / Inheritance and expression of chimeric genes in the progeny of transgenic maize plants. In: Bio/Technology. 1990 ; Vol. 8, No. 9. pp. 833-839.
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