Infrared ellipsometry studies of thermal stability of protein monolayers and multilayers

H. Arwin, A. Askendahl, P. Tengvall, D. W. Thompson, J. A. Woollam

Research output: Contribution to journalConference article

9 Citations (Scopus)

Abstract

Methodology for studies of effects of heating multilayers of human serum albumin (HSA) and anti-HSA is presented. Multilayers of anti-HSA were prepared on silicon substrates and studied with infrared spectroscopic ellipsometry equipped with a heat stage. The refractive index N = n+ik and the layer thickness are determined and the amide bands are analyzed. It is found that HSA/anti-HSA multilayers are stable for shorter times at temperatures above 100 °C, except for small thickness changes. Also pilot studies of effects of heating monolayers of proteins adsorbed on gold substrates is presented.

Original languageEnglish (US)
Pages (from-to)1438-1441
Number of pages4
JournalPhysica Status Solidi (C) Current Topics in Solid State Physics
Volume5
Issue number5
DOIs
StatePublished - Dec 1 2008
Event4th International Conference on Spectroscopic Ellipsometry, ICSE4 - Stockholm, Sweden
Duration: Jun 11 2007Jun 15 2007

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albumins
serums
ellipsometry
thermal stability
proteins
heating
amides
methodology
refractivity
gold
heat
silicon
temperature

ASJC Scopus subject areas

  • Condensed Matter Physics

Cite this

Infrared ellipsometry studies of thermal stability of protein monolayers and multilayers. / Arwin, H.; Askendahl, A.; Tengvall, P.; Thompson, D. W.; Woollam, J. A.

In: Physica Status Solidi (C) Current Topics in Solid State Physics, Vol. 5, No. 5, 01.12.2008, p. 1438-1441.

Research output: Contribution to journalConference article

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