Infrared and visible ellipsometric studies of cholera toxin in ELISA structures

Daniel W. Thompson, Galen Pfeiffer, Emil Berberov, Leon Castro, John A Woollam

Research output: Contribution to journalConference article

1 Citation (Scopus)

Abstract

Ellipsometry is well known for its extreme sensitivity to the presence and properties of ultra-thin films. In the infrared, resonance response to chemical bonds allows chemical identification in monolayer-thick biological films. In this paper we show results of attachment repeatability for successive layers of monosialoganglioside, cholera toxin, and related antibodies using in situ visible spectroscopic ellipsometry. Several factors contributing to difficulty in obtaining reproducible results are discussed. Specifically, these include freshness of reagents; surface type, cleaning, and preparation; temperature; birefringence of liquid cell windows; and cell design. Sensitivity and signal noise considerations for infrared spectra of molecular monolayers are discussed.

Original languageEnglish (US)
Pages (from-to)138-146
Number of pages9
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume4965
DOIs
StatePublished - Dec 5 2003
EventPROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Optical Diagnostics and Sensing in Biomedicine III - San Jose, CA, United States
Duration: Jan 28 2003Jan 29 2003

Fingerprint

cholera
Enzyme-linked Immunosorbent Assay
Cholera Toxin
ellipsometry
Monolayers
Infrared
Spectroscopic Ellipsometry
Infrared radiation
Ellipsometry
Spectroscopic ellipsometry
Ultrathin films
sensitivity
Chemical bonds
Repeatability
Birefringence
Cell
Cleaning
chemical bonds
cells
antibodies

Keywords

  • Biological films
  • Cholera toxin
  • ELISA
  • Ellipsometry

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Cite this

Infrared and visible ellipsometric studies of cholera toxin in ELISA structures. / Thompson, Daniel W.; Pfeiffer, Galen; Berberov, Emil; Castro, Leon; Woollam, John A.

In: Proceedings of SPIE - The International Society for Optical Engineering, Vol. 4965, 05.12.2003, p. 138-146.

Research output: Contribution to journalConference article

@article{da9e36319b93460e9dfb29b4631453e2,
title = "Infrared and visible ellipsometric studies of cholera toxin in ELISA structures",
abstract = "Ellipsometry is well known for its extreme sensitivity to the presence and properties of ultra-thin films. In the infrared, resonance response to chemical bonds allows chemical identification in monolayer-thick biological films. In this paper we show results of attachment repeatability for successive layers of monosialoganglioside, cholera toxin, and related antibodies using in situ visible spectroscopic ellipsometry. Several factors contributing to difficulty in obtaining reproducible results are discussed. Specifically, these include freshness of reagents; surface type, cleaning, and preparation; temperature; birefringence of liquid cell windows; and cell design. Sensitivity and signal noise considerations for infrared spectra of molecular monolayers are discussed.",
keywords = "Biological films, Cholera toxin, ELISA, Ellipsometry",
author = "Thompson, {Daniel W.} and Galen Pfeiffer and Emil Berberov and Leon Castro and Woollam, {John A}",
year = "2003",
month = "12",
day = "5",
doi = "10.1117/12.479262",
language = "English (US)",
volume = "4965",
pages = "138--146",
journal = "Proceedings of SPIE - The International Society for Optical Engineering",
issn = "0277-786X",
publisher = "SPIE",

}

TY - JOUR

T1 - Infrared and visible ellipsometric studies of cholera toxin in ELISA structures

AU - Thompson, Daniel W.

AU - Pfeiffer, Galen

AU - Berberov, Emil

AU - Castro, Leon

AU - Woollam, John A

PY - 2003/12/5

Y1 - 2003/12/5

N2 - Ellipsometry is well known for its extreme sensitivity to the presence and properties of ultra-thin films. In the infrared, resonance response to chemical bonds allows chemical identification in monolayer-thick biological films. In this paper we show results of attachment repeatability for successive layers of monosialoganglioside, cholera toxin, and related antibodies using in situ visible spectroscopic ellipsometry. Several factors contributing to difficulty in obtaining reproducible results are discussed. Specifically, these include freshness of reagents; surface type, cleaning, and preparation; temperature; birefringence of liquid cell windows; and cell design. Sensitivity and signal noise considerations for infrared spectra of molecular monolayers are discussed.

AB - Ellipsometry is well known for its extreme sensitivity to the presence and properties of ultra-thin films. In the infrared, resonance response to chemical bonds allows chemical identification in monolayer-thick biological films. In this paper we show results of attachment repeatability for successive layers of monosialoganglioside, cholera toxin, and related antibodies using in situ visible spectroscopic ellipsometry. Several factors contributing to difficulty in obtaining reproducible results are discussed. Specifically, these include freshness of reagents; surface type, cleaning, and preparation; temperature; birefringence of liquid cell windows; and cell design. Sensitivity and signal noise considerations for infrared spectra of molecular monolayers are discussed.

KW - Biological films

KW - Cholera toxin

KW - ELISA

KW - Ellipsometry

UR - http://www.scopus.com/inward/record.url?scp=0345714693&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0345714693&partnerID=8YFLogxK

U2 - 10.1117/12.479262

DO - 10.1117/12.479262

M3 - Conference article

VL - 4965

SP - 138

EP - 146

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

SN - 0277-786X

ER -