Infrared and visible ellipsometric studies of cholera toxin in ELISA structures

Daniel W. Thompson, Galen Pfeiffer, Emil Berberov, Leon Castro, John A. Woollam

Research output: Contribution to journalConference article

1 Scopus citations

Abstract

Ellipsometry is well known for its extreme sensitivity to the presence and properties of ultra-thin films. In the infrared, resonance response to chemical bonds allows chemical identification in monolayer-thick biological films. In this paper we show results of attachment repeatability for successive layers of monosialoganglioside, cholera toxin, and related antibodies using in situ visible spectroscopic ellipsometry. Several factors contributing to difficulty in obtaining reproducible results are discussed. Specifically, these include freshness of reagents; surface type, cleaning, and preparation; temperature; birefringence of liquid cell windows; and cell design. Sensitivity and signal noise considerations for infrared spectra of molecular monolayers are discussed.

Original languageEnglish (US)
Pages (from-to)138-146
Number of pages9
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume4965
DOIs
StatePublished - Dec 5 2003
EventPROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Optical Diagnostics and Sensing in Biomedicine III - San Jose, CA, United States
Duration: Jan 28 2003Jan 29 2003

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Keywords

  • Biological films
  • Cholera toxin
  • ELISA
  • Ellipsometry

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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