Influence of trinexapac-ethyl on respiration of isolated wheat mitochondria

Neil L. Heckman, Thomas E. Elthon, Garald L. Horst, Roch E. Gaussoin

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The plant growth regulators (PGRs) 2,4-D [(2,4-dichlorophenoxy) acetic acid] and malic hydrazide (1,2-dyhydro-3,6-pyridazinedione) have been shown to reduce plant respiration. The effect of other PGRs such as trinexapac-ethyl [(4-cyclopropyl-α-hydroxy-methylene)-3,5-dioxocyclohexanecarboxylic acid methyl ester] on respiration is unknown. Experiments were conducted to evaluate the effects of trinexapac-ethyl and 2-oxoglutarate on the respiration of mitochondria isolated from wheat (Triticum aestivum 'Arapahoe') seedlings. Trinexapac-ethyl applied at increasing concentrations inhibited nicotinamide adenine dinucleotide (NADH)-dependent O uptake, while 2-oxoglutarate had no significant effect. This is different than other reports that trinexapac-ethyl may compete with 2-oxoglutarate for binding sites. Different regions of the mitochondria were tested to determine the site of inhibition caused by trinexapac-ethyl. Nicotinamide adenine dinucleotide dehydrogenase activity, duroquinol-dependent O uptake, and cytochrome bc1 activity were all reduced by ≅30% in the presence of 10 mM trinexapac-ethyl. Succinate-dependent O uptake, alternative oxidase, and cytochrome oxidase were not reduced by any trinexapac-ethyl concentration. This revealed possible interference of trinexapac-ethyl with ubiquinone binding sites. The reduced form of trinexapac-ethyl was observed to inhibit the electron transport chain greater than the oxidized form. Reduction in respiration from trinexapac-ethyl may result in greater stress tolerance in treated plants.

Original languageEnglish (US)
Pages (from-to)423-427
Number of pages5
JournalCrop Science
Volume42
Issue number2
StatePublished - Jul 14 2002

Fingerprint

cell respiration
NAD (coenzyme)
mitochondria
wheat
uptake mechanisms
plant growth substances
2,4-D
binding sites
hydrazides
ubiquinones
electron transport chain
succinic acid
cytochromes
stress tolerance
cytochrome-c oxidase
Triticum aestivum
esters
seedlings
acids

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Heckman, N. L., Elthon, T. E., Horst, G. L., & Gaussoin, R. E. (2002). Influence of trinexapac-ethyl on respiration of isolated wheat mitochondria. Crop Science, 42(2), 423-427.

Influence of trinexapac-ethyl on respiration of isolated wheat mitochondria. / Heckman, Neil L.; Elthon, Thomas E.; Horst, Garald L.; Gaussoin, Roch E.

In: Crop Science, Vol. 42, No. 2, 14.07.2002, p. 423-427.

Research output: Contribution to journalArticle

Heckman, NL, Elthon, TE, Horst, GL & Gaussoin, RE 2002, 'Influence of trinexapac-ethyl on respiration of isolated wheat mitochondria', Crop Science, vol. 42, no. 2, pp. 423-427.
Heckman NL, Elthon TE, Horst GL, Gaussoin RE. Influence of trinexapac-ethyl on respiration of isolated wheat mitochondria. Crop Science. 2002 Jul 14;42(2):423-427.
Heckman, Neil L. ; Elthon, Thomas E. ; Horst, Garald L. ; Gaussoin, Roch E. / Influence of trinexapac-ethyl on respiration of isolated wheat mitochondria. In: Crop Science. 2002 ; Vol. 42, No. 2. pp. 423-427.
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