Influence of oxidative damage to proteins on meat tenderness using a proteomics approach

Jessica Moraes Malheiros, Camila Pereira Braga, Ryan Albert Grove, Felipe Azevedo Ribeiro, Chris Richard Calkins, Jiri Adamec, Luis Artur Loyola Chardulo

Research output: Contribution to journalArticle

Abstract

The objective of this study was to evaluate the association between oxidative damage to proteins (represented by protein carbonylation) and beef tenderness. Three experimental groups were selected by shear force (SF): tender (38.2 ± 2.9 N), intermediate (51.9 ± 6.8 N), and tough meat (74.5 ± 7.8 N). Two-dimensional electrophoresis with hydrazide fluorophore derivatization was used. The structural proteins actin (ACTA1), myosin (MYL1 and MYL3), desmin (DES) and troponin T (TNNT1 and TNNT3), antioxidant proteins (PRDX1, PRDX2 and PARK7) and heat shock proteins (HSPB1, CRYAB and HSPB6) showed an increase in the oxidative damage in tender meat when compared to the intermediate and tough meat (P <.05). Decrease in oxidative damage of the metabolic enzymes (TPI1, GAPDH and ENO3) were observed in tender meat group (P <.05). The present results suggest that oxidation act on the proteins of different metabolic pathways and consequently affect meat tenderness in Angus crossbred cattle.

LanguageEnglish (US)
Pages64-71
Number of pages8
JournalMeat Science
Volume148
DOIs
StatePublished - Feb 1 2019

Fingerprint

meat tenderness
Proteomics
proteomics
Meat
meat
Proteins
proteins
hydrazides
troponin T
desmin
Protein Carbonylation
fluorescent dyes
structural proteins
derivatization
Angus
myosin
heat shock proteins
Troponin T
Desmin
actin

Keywords

  • 2D-DIGE
  • Beef cattle
  • Protein oxidation
  • Shear force

ASJC Scopus subject areas

  • Food Science

Cite this

Malheiros, J. M., Braga, C. P., Grove, R. A., Ribeiro, F. A., Calkins, C. R., Adamec, J., & Chardulo, L. A. L. (2019). Influence of oxidative damage to proteins on meat tenderness using a proteomics approach. Meat Science, 148, 64-71. https://doi.org/10.1016/j.meatsci.2018.08.016

Influence of oxidative damage to proteins on meat tenderness using a proteomics approach. / Malheiros, Jessica Moraes; Braga, Camila Pereira; Grove, Ryan Albert; Ribeiro, Felipe Azevedo; Calkins, Chris Richard; Adamec, Jiri; Chardulo, Luis Artur Loyola.

In: Meat Science, Vol. 148, 01.02.2019, p. 64-71.

Research output: Contribution to journalArticle

Malheiros, JM, Braga, CP, Grove, RA, Ribeiro, FA, Calkins, CR, Adamec, J & Chardulo, LAL 2019, 'Influence of oxidative damage to proteins on meat tenderness using a proteomics approach' Meat Science, vol. 148, pp. 64-71. https://doi.org/10.1016/j.meatsci.2018.08.016
Malheiros, Jessica Moraes ; Braga, Camila Pereira ; Grove, Ryan Albert ; Ribeiro, Felipe Azevedo ; Calkins, Chris Richard ; Adamec, Jiri ; Chardulo, Luis Artur Loyola. / Influence of oxidative damage to proteins on meat tenderness using a proteomics approach. In: Meat Science. 2019 ; Vol. 148. pp. 64-71.
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