Influence of organ site and tumor cell type on MUC1-specific tumor immunity

Keita Morikane, Richard M Tempero, Connie L. Sivinski, Shimichi Kitajima, Sandra J. Gendler, Michael A Hollingsworth

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We investigated the influence of organ-specific parameters on tolerance and immunity to human MUC1. C57BI/6 mice (wild-type) and C57BI/6 transgenic for MUC1 (MUC1. Tg) were challenged in the pancreas with Panc02-MUC1, a C57B1/6-syngeneic pancreatic cancer cell line expressing human MUC1. Wild-type mice produced immune responses to MUC1 when presented on tumor cells growing in the pancreas; however, the responses to tumors in the pancreas were less effective than responses produced by tumor challenge at the s.c. site. Tumor immunity specific for MUC1 was produced in wild-type mice by two different procedures: (i) s.c. immunization of wild-type mice with a low dose of PancO2-MUC1 or (ii) adoptive transfer of spleen and lymph node cells harvested from wild-type mice previously immunized s.c. with Panc02-MUC1. This demonstrates that immune responses to MUC1 presented at the s.c. site can be detected and adoptively transferred. MUC1.Tg mice were immunologically tolerant to MUC1; however, some immunological protection against orthotopic challenge with PancO2-MUC1 was conferred by adoptive transfer of CD4+ and CD8+ T cells from wild-type mice. These results show that it is more difficult to produce immune responses to tumors growing at the pancreatic site than the s.c. site. PancO2-MUC1 cells growing in the pancreas were accessible to the immune system, and immune responses evoked by s.c. presentation of this molecule in wild-type mice were effective in rejecting tumor cells in the pancreas of both wild-type and MUC1.Tg mice. No effective anti -tumor immune responses against MUC1 were produced in MUC1.Tg mice.

Original languageEnglish (US)
Pages (from-to)233-240
Number of pages8
JournalInternational Immunology
Volume13
Issue number2
DOIs
StatePublished - Jan 1 2001

Fingerprint

Immunity
Pancreas
Neoplasms
Adoptive Transfer
Pancreatic Neoplasms
Immune System
Immunization
Spleen
Lymph Nodes
T-Lymphocytes
Cell Line

Keywords

  • Immunological tolerance
  • MUC1
  • Orthotopic
  • Pancreatic cancer
  • Tumor immunity

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Influence of organ site and tumor cell type on MUC1-specific tumor immunity. / Morikane, Keita; Tempero, Richard M; Sivinski, Connie L.; Kitajima, Shimichi; Gendler, Sandra J.; Hollingsworth, Michael A.

In: International Immunology, Vol. 13, No. 2, 01.01.2001, p. 233-240.

Research output: Contribution to journalArticle

Morikane, Keita ; Tempero, Richard M ; Sivinski, Connie L. ; Kitajima, Shimichi ; Gendler, Sandra J. ; Hollingsworth, Michael A. / Influence of organ site and tumor cell type on MUC1-specific tumor immunity. In: International Immunology. 2001 ; Vol. 13, No. 2. pp. 233-240.
@article{3d664a37b0214d928c56a7effc69c4cb,
title = "Influence of organ site and tumor cell type on MUC1-specific tumor immunity",
abstract = "We investigated the influence of organ-specific parameters on tolerance and immunity to human MUC1. C57BI/6 mice (wild-type) and C57BI/6 transgenic for MUC1 (MUC1. Tg) were challenged in the pancreas with Panc02-MUC1, a C57B1/6-syngeneic pancreatic cancer cell line expressing human MUC1. Wild-type mice produced immune responses to MUC1 when presented on tumor cells growing in the pancreas; however, the responses to tumors in the pancreas were less effective than responses produced by tumor challenge at the s.c. site. Tumor immunity specific for MUC1 was produced in wild-type mice by two different procedures: (i) s.c. immunization of wild-type mice with a low dose of PancO2-MUC1 or (ii) adoptive transfer of spleen and lymph node cells harvested from wild-type mice previously immunized s.c. with Panc02-MUC1. This demonstrates that immune responses to MUC1 presented at the s.c. site can be detected and adoptively transferred. MUC1.Tg mice were immunologically tolerant to MUC1; however, some immunological protection against orthotopic challenge with PancO2-MUC1 was conferred by adoptive transfer of CD4+ and CD8+ T cells from wild-type mice. These results show that it is more difficult to produce immune responses to tumors growing at the pancreatic site than the s.c. site. PancO2-MUC1 cells growing in the pancreas were accessible to the immune system, and immune responses evoked by s.c. presentation of this molecule in wild-type mice were effective in rejecting tumor cells in the pancreas of both wild-type and MUC1.Tg mice. No effective anti -tumor immune responses against MUC1 were produced in MUC1.Tg mice.",
keywords = "Immunological tolerance, MUC1, Orthotopic, Pancreatic cancer, Tumor immunity",
author = "Keita Morikane and Tempero, {Richard M} and Sivinski, {Connie L.} and Shimichi Kitajima and Gendler, {Sandra J.} and Hollingsworth, {Michael A}",
year = "2001",
month = "1",
day = "1",
doi = "10.1093/intimm/13.2.233",
language = "English (US)",
volume = "13",
pages = "233--240",
journal = "International Immunology",
issn = "0953-8178",
publisher = "Oxford University Press",
number = "2",

}

TY - JOUR

T1 - Influence of organ site and tumor cell type on MUC1-specific tumor immunity

AU - Morikane, Keita

AU - Tempero, Richard M

AU - Sivinski, Connie L.

AU - Kitajima, Shimichi

AU - Gendler, Sandra J.

AU - Hollingsworth, Michael A

PY - 2001/1/1

Y1 - 2001/1/1

N2 - We investigated the influence of organ-specific parameters on tolerance and immunity to human MUC1. C57BI/6 mice (wild-type) and C57BI/6 transgenic for MUC1 (MUC1. Tg) were challenged in the pancreas with Panc02-MUC1, a C57B1/6-syngeneic pancreatic cancer cell line expressing human MUC1. Wild-type mice produced immune responses to MUC1 when presented on tumor cells growing in the pancreas; however, the responses to tumors in the pancreas were less effective than responses produced by tumor challenge at the s.c. site. Tumor immunity specific for MUC1 was produced in wild-type mice by two different procedures: (i) s.c. immunization of wild-type mice with a low dose of PancO2-MUC1 or (ii) adoptive transfer of spleen and lymph node cells harvested from wild-type mice previously immunized s.c. with Panc02-MUC1. This demonstrates that immune responses to MUC1 presented at the s.c. site can be detected and adoptively transferred. MUC1.Tg mice were immunologically tolerant to MUC1; however, some immunological protection against orthotopic challenge with PancO2-MUC1 was conferred by adoptive transfer of CD4+ and CD8+ T cells from wild-type mice. These results show that it is more difficult to produce immune responses to tumors growing at the pancreatic site than the s.c. site. PancO2-MUC1 cells growing in the pancreas were accessible to the immune system, and immune responses evoked by s.c. presentation of this molecule in wild-type mice were effective in rejecting tumor cells in the pancreas of both wild-type and MUC1.Tg mice. No effective anti -tumor immune responses against MUC1 were produced in MUC1.Tg mice.

AB - We investigated the influence of organ-specific parameters on tolerance and immunity to human MUC1. C57BI/6 mice (wild-type) and C57BI/6 transgenic for MUC1 (MUC1. Tg) were challenged in the pancreas with Panc02-MUC1, a C57B1/6-syngeneic pancreatic cancer cell line expressing human MUC1. Wild-type mice produced immune responses to MUC1 when presented on tumor cells growing in the pancreas; however, the responses to tumors in the pancreas were less effective than responses produced by tumor challenge at the s.c. site. Tumor immunity specific for MUC1 was produced in wild-type mice by two different procedures: (i) s.c. immunization of wild-type mice with a low dose of PancO2-MUC1 or (ii) adoptive transfer of spleen and lymph node cells harvested from wild-type mice previously immunized s.c. with Panc02-MUC1. This demonstrates that immune responses to MUC1 presented at the s.c. site can be detected and adoptively transferred. MUC1.Tg mice were immunologically tolerant to MUC1; however, some immunological protection against orthotopic challenge with PancO2-MUC1 was conferred by adoptive transfer of CD4+ and CD8+ T cells from wild-type mice. These results show that it is more difficult to produce immune responses to tumors growing at the pancreatic site than the s.c. site. PancO2-MUC1 cells growing in the pancreas were accessible to the immune system, and immune responses evoked by s.c. presentation of this molecule in wild-type mice were effective in rejecting tumor cells in the pancreas of both wild-type and MUC1.Tg mice. No effective anti -tumor immune responses against MUC1 were produced in MUC1.Tg mice.

KW - Immunological tolerance

KW - MUC1

KW - Orthotopic

KW - Pancreatic cancer

KW - Tumor immunity

UR - http://www.scopus.com/inward/record.url?scp=0035146350&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035146350&partnerID=8YFLogxK

U2 - 10.1093/intimm/13.2.233

DO - 10.1093/intimm/13.2.233

M3 - Article

VL - 13

SP - 233

EP - 240

JO - International Immunology

JF - International Immunology

SN - 0953-8178

IS - 2

ER -