Influence of bilirubin on human platelets

Harold M. Maurer, Joyce Caul

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Studies were performed which showed that unbound unconjugated bilirubin at low concentration (0.5 mg/100 ml) causes yellow staining and aggregation of washed human platelets, whereas bilirubin bound to albumin and bilirubin altered by exposure to light have little, if any, of these effects on platelets. Less intense aggregation occurred in some but not all samples of platelet-rich plasma incubated with bilirubin and only at bilirubin levels of 10 mg/100 ml or higher. Aggregation of platelets by bilirubin showed an absolute requirement for calcium ions and this response was enhanced by potassium ions. Adenosine diphosphate and adenosine triphosphate were released from platelets with bilirubin. Speculation: In the early accounts of human kernicterus, and in studies of experimental hyperbilirubinemia in animals, hemorrhagic complications were well known but poorly understood. The data presented suggest that bilirubin influences platelet function. It is tempting to speculate that the effect of bilirubin on platelets could play a role in producing intravascular thrombosis or hemorrhage, or both, in severe hyperbilirubinemia.

Original languageEnglish (US)
Pages (from-to)136-144
Number of pages9
JournalPediatric Research
Volume6
Issue number2
DOIs
StatePublished - Feb 1972

Fingerprint

Bilirubin
Blood Platelets
Hyperbilirubinemia
Kernicterus
Ions
Platelet-Rich Plasma
Platelet Aggregation
Adenosine Diphosphate
Albumins
Potassium
Thrombosis
Adenosine Triphosphate
Staining and Labeling
Hemorrhage
Calcium
Light

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Influence of bilirubin on human platelets. / Maurer, Harold M.; Caul, Joyce.

In: Pediatric Research, Vol. 6, No. 2, 02.1972, p. 136-144.

Research output: Contribution to journalArticle

Maurer, Harold M. ; Caul, Joyce. / Influence of bilirubin on human platelets. In: Pediatric Research. 1972 ; Vol. 6, No. 2. pp. 136-144.
@article{42c5deefa5a2439ea3389770b838236d,
title = "Influence of bilirubin on human platelets",
abstract = "Studies were performed which showed that unbound unconjugated bilirubin at low concentration (0.5 mg/100 ml) causes yellow staining and aggregation of washed human platelets, whereas bilirubin bound to albumin and bilirubin altered by exposure to light have little, if any, of these effects on platelets. Less intense aggregation occurred in some but not all samples of platelet-rich plasma incubated with bilirubin and only at bilirubin levels of 10 mg/100 ml or higher. Aggregation of platelets by bilirubin showed an absolute requirement for calcium ions and this response was enhanced by potassium ions. Adenosine diphosphate and adenosine triphosphate were released from platelets with bilirubin. Speculation: In the early accounts of human kernicterus, and in studies of experimental hyperbilirubinemia in animals, hemorrhagic complications were well known but poorly understood. The data presented suggest that bilirubin influences platelet function. It is tempting to speculate that the effect of bilirubin on platelets could play a role in producing intravascular thrombosis or hemorrhage, or both, in severe hyperbilirubinemia.",
author = "Maurer, {Harold M.} and Joyce Caul",
year = "1972",
month = "2",
doi = "10.1203/00006450-197202000-00007",
language = "English (US)",
volume = "6",
pages = "136--144",
journal = "Pediatric Research",
issn = "0031-3998",
publisher = "Lippincott Williams and Wilkins",
number = "2",

}

TY - JOUR

T1 - Influence of bilirubin on human platelets

AU - Maurer, Harold M.

AU - Caul, Joyce

PY - 1972/2

Y1 - 1972/2

N2 - Studies were performed which showed that unbound unconjugated bilirubin at low concentration (0.5 mg/100 ml) causes yellow staining and aggregation of washed human platelets, whereas bilirubin bound to albumin and bilirubin altered by exposure to light have little, if any, of these effects on platelets. Less intense aggregation occurred in some but not all samples of platelet-rich plasma incubated with bilirubin and only at bilirubin levels of 10 mg/100 ml or higher. Aggregation of platelets by bilirubin showed an absolute requirement for calcium ions and this response was enhanced by potassium ions. Adenosine diphosphate and adenosine triphosphate were released from platelets with bilirubin. Speculation: In the early accounts of human kernicterus, and in studies of experimental hyperbilirubinemia in animals, hemorrhagic complications were well known but poorly understood. The data presented suggest that bilirubin influences platelet function. It is tempting to speculate that the effect of bilirubin on platelets could play a role in producing intravascular thrombosis or hemorrhage, or both, in severe hyperbilirubinemia.

AB - Studies were performed which showed that unbound unconjugated bilirubin at low concentration (0.5 mg/100 ml) causes yellow staining and aggregation of washed human platelets, whereas bilirubin bound to albumin and bilirubin altered by exposure to light have little, if any, of these effects on platelets. Less intense aggregation occurred in some but not all samples of platelet-rich plasma incubated with bilirubin and only at bilirubin levels of 10 mg/100 ml or higher. Aggregation of platelets by bilirubin showed an absolute requirement for calcium ions and this response was enhanced by potassium ions. Adenosine diphosphate and adenosine triphosphate were released from platelets with bilirubin. Speculation: In the early accounts of human kernicterus, and in studies of experimental hyperbilirubinemia in animals, hemorrhagic complications were well known but poorly understood. The data presented suggest that bilirubin influences platelet function. It is tempting to speculate that the effect of bilirubin on platelets could play a role in producing intravascular thrombosis or hemorrhage, or both, in severe hyperbilirubinemia.

UR - http://www.scopus.com/inward/record.url?scp=0015298033&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0015298033&partnerID=8YFLogxK

U2 - 10.1203/00006450-197202000-00007

DO - 10.1203/00006450-197202000-00007

M3 - Article

C2 - 5047472

AN - SCOPUS:0015298033

VL - 6

SP - 136

EP - 144

JO - Pediatric Research

JF - Pediatric Research

SN - 0031-3998

IS - 2

ER -