Influence of animal origin and lineage on survival of Escherichia coli O157:H7 strains in strong and weak acid challenges

Constantine E. Saridakis, Roger P. Johnson, Andrew Benson, Kim Ziebell, Carlton L. Gyles

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Twenty-five strains of Escherichia coli O157:H7 isolated from humans, cattle, and pigs were maintained in HCl (pH 2.5) and in a volatile fatty acid (VFA) mixture (pH 4.0) for up to 6 h at 37°C to assess their ability to survive in acidic conditions that simulate those of the stomach and ileum, respectively. In HCl, the average group survival of bovine strains was significantly higher than that of porcine and human strains, whereas in VFAs, porcine strains were significantly more resistant than bovine and human strains. Bovine strains exhibited significantly higher average survival in HCl than in VFAs. The average survival of strains classified as octamer-based genome scanning (OBGS) lineage II was significantly superior to that of strains classified as OBGS lineage I in HCl. The group of lineage I strains was more resistant in VFAs compared with lineage II, but only after 6 h of challenge. The possible involvement of urease in acid resistance of E. coli O157:H7 was also examined. Although the strains possessed the ureC gene, as shown by PCR, this gene did not appear to contribute to acid resistance under the conditions tested. The data indicate that there is a relationship between acid resistance and source or lineage of O157:H7 strains.

Original languageEnglish (US)
Pages (from-to)1591-1596
Number of pages6
JournalJournal of food protection
Volume67
Issue number8
DOIs
StatePublished - Aug 2004

Fingerprint

Escherichia coli O157
Acids
Swine
Survival
acids
animals
Genome
acid tolerance
Urease
Volatile Fatty Acids
Ileum
Genes
Stomach
cattle
swine
Polymerase Chain Reaction
genome
urease
volatile fatty acids
ileum

ASJC Scopus subject areas

  • Food Science
  • Microbiology

Cite this

Influence of animal origin and lineage on survival of Escherichia coli O157:H7 strains in strong and weak acid challenges. / Saridakis, Constantine E.; Johnson, Roger P.; Benson, Andrew; Ziebell, Kim; Gyles, Carlton L.

In: Journal of food protection, Vol. 67, No. 8, 08.2004, p. 1591-1596.

Research output: Contribution to journalArticle

Saridakis, Constantine E. ; Johnson, Roger P. ; Benson, Andrew ; Ziebell, Kim ; Gyles, Carlton L. / Influence of animal origin and lineage on survival of Escherichia coli O157:H7 strains in strong and weak acid challenges. In: Journal of food protection. 2004 ; Vol. 67, No. 8. pp. 1591-1596.
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