Inductively coupled mass spectrometry analysis of biometals in conditional Hamp1 and Hamp1 and Hamp2 transgenic mouse models

S. Lu, Javier Seravalli, D. Harrison-Findik

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Hepcidin, a circulatory antimicrobial peptide, is involved in iron homeostasis, inflammation, infection and metabolic signals. Humans express one hepcidin gene, HAMP but mice express two hepcidin genes, Hamp1 and Hamp2. Consecutive gene targeting events were performed to produce transgenic mice expressing conditional alleles of either Hamp1 or both Hamp1 and Hamp2 (Hamp1/2). The deletion of Hamp1 alleles elevated Hamp2 expression, particularly in males, which was reduced by endotoxin treatment. The tissue levels of iron and other biometals were quantified by inductively coupled mass spectrometry. The ubiquitous or liver-specific deletion of Hamp1 alleles yielded similar quantitative changes in iron levels in the liver, duodenum, spleen, kidney, heart and brain. The introduction of Hamp2 null allele did not exacerbate the iron-related phenotype of Hamp1 null allele. Besides iron, Hamp1 null allele significantly elevated the levels of selenium in the liver, manganese in the liver and duodenum, and copper in the brain. Mice with conditional Hamp alleles will be useful to determine the tissue-specific regulation and functions of Hamp1 and Hamp2 in biometal homeostasis and other biological processes.

Original languageEnglish (US)
Pages (from-to)765-773
Number of pages9
JournalTransgenic Research
Volume24
Issue number4
DOIs
StatePublished - Apr 23 2015

Fingerprint

Trace Elements
Transgenic Mice
Mass Spectrometry
animal models
Alleles
genetically modified organisms
mass spectrometry
null alleles
iron
Hepcidins
Iron
alleles
liver
duodenum
homeostasis
mice
Liver
Duodenum
brain
Homeostasis

Keywords

  • Copper
  • Hepcidin
  • Iron
  • Liver
  • Manganese
  • Selenium

ASJC Scopus subject areas

  • Biotechnology
  • Genetics
  • Agronomy and Crop Science
  • Animal Science and Zoology

Cite this

Inductively coupled mass spectrometry analysis of biometals in conditional Hamp1 and Hamp1 and Hamp2 transgenic mouse models. / Lu, S.; Seravalli, Javier; Harrison-Findik, D.

In: Transgenic Research, Vol. 24, No. 4, 23.04.2015, p. 765-773.

Research output: Contribution to journalArticle

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