Induction of stress granule-like structures in vesicular stomatitis virus-infected cells

Phat X. Dinh, Lalit K. Beura, Phani B. Das, Debasis Panda, Anshuman Das, Asit K Pattnaik

Research output: Contribution to journalArticle

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Abstract

Previous studies from our laboratory revealed that cellular poly(C) binding protein 2 (PCBP2) downregulates vesicular stomatitis virus (VSV) gene expression. We show here that VSV infection induces the formation of granular structures in the cytoplasm containing cellular RNA-binding proteins, including PCBP2, T-cell-restricted intracellular antigen 1 (TIA1), and TIA1-related protein (TIAR). Depletion of TIA1 via small interfering RNAs (siRNAs), but not depletion of TIAR, results in enhanced VSV growth and gene expression. The VSV-induced granules appear to be similar to the stress granules (SGs) generated in cells triggered by heat shock or oxidative stress but do not contain some of the bona fide SG markers, such as eukaryotic initiation factor 3 (eIF3) or eIF4A, or the processing body (PB) markers, such as mRNA-decapping enzyme 1A (DCP1a), and thus may not represent canonical SGs or PBs. Our results revealed that the VSV-induced granules, called SG-like structures here, contain the viral replicative proteins and RNAs. The formation and maintenance of the SG-like structures required viral replication and ongoing protein synthesis, but an intact cytoskeletal network was not necessary. These results suggest that cells respond to VSV infection by aggregating the antiviral proteins, such as PCBP2 and TIA1, to form SG-like structures. The functional significance of these SG-like structures in VSV-infected cells is currently under investigation.

Original languageEnglish (US)
Pages (from-to)372-383
Number of pages12
JournalJournal of virology
Volume87
Issue number1
DOIs
StatePublished - Jan 1 2013

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Vesiculovirus
Vesicular Stomatitis
granules
Viruses
Poly C
T-Lymphocytes
Antigens
Carrier Proteins
cells
T-lymphocytes
Virus Diseases
antigens
binding proteins
Eukaryotic Initiation Factor-3
Proteins
Gene Expression
Viral Structures
RNA-Binding Proteins
antiviral proteins
Viral RNA

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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Induction of stress granule-like structures in vesicular stomatitis virus-infected cells. / Dinh, Phat X.; Beura, Lalit K.; Das, Phani B.; Panda, Debasis; Das, Anshuman; Pattnaik, Asit K.

In: Journal of virology, Vol. 87, No. 1, 01.01.2013, p. 372-383.

Research output: Contribution to journalArticle

Dinh, Phat X. ; Beura, Lalit K. ; Das, Phani B. ; Panda, Debasis ; Das, Anshuman ; Pattnaik, Asit K. / Induction of stress granule-like structures in vesicular stomatitis virus-infected cells. In: Journal of virology. 2013 ; Vol. 87, No. 1. pp. 372-383.
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