Induction of neutralizing antibodies to transmissible gastroenteritis virus by anti-idiotypic antibodies

K. Hariharan, S. Srikumaran, R. A. Moxley, F. A. Osorio, A. A. Morales

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The potential of anti-idiotypic antibodies (anti-ids) as immunogens against transmissible gastroenteritis virus (TGEV) was tested in a heterologous system. A month-old pig was immunized with a neutralizing murine monoclonal antibody (MAb, 5A5) of the IgG(2a) isotype, specific for the E2 glycoprotein of TGEV. The anti-ids were isolated from the serum of the immunized pig by affinity chromatography, initially on a 5A5-Sepharose column, followed by repeated adsorption on a mouse IgG(2a) column. The swine anti-ids thus obtained bound to the MAb 5A5 (the idiotype), but not to MAbs of the same isotype IgG(2a) but of different idiotypes. The anti-ids also inhibited the binding of 5A5 to TGEV in a concentration-dependent manner. Mice immunized with the anti-ids produced antibodies to TGEV. These antibodies, neutralized TGEV in vitro and inhibited the binding of 5A5 to TGEV.

Original languageEnglish (US)
Pages (from-to)133-142
Number of pages10
JournalViral Immunology
Volume2
Issue number2
DOIs
StatePublished - Jan 1 1989

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Transmissible gastroenteritis virus
Neutralizing Antibodies
Anti-Idiotypic Antibodies
Swine
Immunoglobulin G
Antibodies
Affinity Chromatography
Sepharose
Adsorption
Glycoproteins
Monoclonal Antibodies
Serum

ASJC Scopus subject areas

  • Immunology
  • Molecular Medicine
  • Virology

Cite this

Induction of neutralizing antibodies to transmissible gastroenteritis virus by anti-idiotypic antibodies. / Hariharan, K.; Srikumaran, S.; Moxley, R. A.; Osorio, F. A.; Morales, A. A.

In: Viral Immunology, Vol. 2, No. 2, 01.01.1989, p. 133-142.

Research output: Contribution to journalArticle

Hariharan, K. ; Srikumaran, S. ; Moxley, R. A. ; Osorio, F. A. ; Morales, A. A. / Induction of neutralizing antibodies to transmissible gastroenteritis virus by anti-idiotypic antibodies. In: Viral Immunology. 1989 ; Vol. 2, No. 2. pp. 133-142.
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