Induction of human mammary-associated serum amyloid A3 expression by prolactin or lipopolysaccharide

Marilynn A. Larson, Shu H. Wei, Annika Weber, Allen T. Weber, Thomas L. McDonald

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

In most mammalian species, serum amyloid A isoform 3 (SAA3) appears to be the predominant SAA isoform expressed extrahepatically. However, human SAA3 gene expression has not been detected previously and, therefore, this gene was referred to as a pseudogene. We report for the first time the transcriptional expression of human SAA3. Human SAA3 gene expression was detected by RT-PCR after stimulation of mammary gland epithelial cells with either prolactin (PRL) or lipopolysaccharide (LPS). The full-length 655bp cDNA sequence for this mammary-associated serum amyloid A3 (M-SAA3) was obtained using 5′ and 3′ rapid amplification of cDNA ends (RACE). The human M-SAA3 transcript would conceptually translate into a 42 residue mature protein, which is smaller than other mammalian SAA3 isoforms that are typically 104-113 amino acids in length. This study defines the cDNA sequence for human SAA3 and also demonstrates the upregulation of M-SAA3 expression in response to the lactational hormone PRL or to an acute phase stimulant such as LPS.

Original languageEnglish (US)
Pages (from-to)1030-1037
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume301
Issue number4
DOIs
Publication statusPublished - Feb 21 2003

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Keywords

  • Human mammary-associated serum amyloid A3
  • Lipopolysaccharide
  • Mammary gland
  • Prolactin
  • cDNA

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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