Induction of experimental autoimmune sialoadenitis by immunization of PL/J mice with carbonic anhydrase II

I. Nishimori, T. Bratanova, I. Toshkov, T. Caffrey, M. Mogaki, Y. Shibata, Michael A Hollingsworth

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

Experimental autoimmune sialoadenitis was induced in PL/J (H-2(u)) mice by intradermal immunization with human carbonic anhydrase II (CAII) and adjuvant containing monophosphoryl lipid A and trehalose diorynomycolate. Mice immunized with CAII showed a significant increase in the number and size of loci with lymphocytic infiltration in the salivary gland compared with mice immunized with adjuvant alone and untreated mice. In mice immunized with CAII, lymphocytic loci were observed around intercalated and intralobular ducts in the salivary glands, resulting in atrophy and replacement of acinar units. The epithelial cells of salivary ducts adjacent to the lymphocytic foci showed both degenerative and regenerative changes. Similar lymphocytic infiltrations were observed in the pancreas and kidney of a few mice immunized with CAII. Among several mouse strains with different H-2 haplotypes (p, q, r, s, and u), strains bearing H-2(s) and H-2(u) were susceptible to CAII-induced sialoadenitis. These results indicate that sialoadenitis induced by the immunization of CAII in mice may serve as a disease model of Sjogren's syndrome and that CAII or its derived peptides in association with the MHC may be one Ag recognized by an autoimmune response in this syndrome.

Original languageEnglish (US)
Pages (from-to)4865-4873
Number of pages9
JournalJournal of Immunology
Volume154
Issue number9
StatePublished - Jan 1 1995
Externally publishedYes

Fingerprint

Sialadenitis
Carbonic Anhydrase II
Immunization
Salivary Glands
Salivary Ducts
Trehalose
Sjogren's Syndrome
Autoimmunity
Haplotypes
Atrophy
Pancreas
Epithelial Cells
Kidney
Peptides

ASJC Scopus subject areas

  • Immunology

Cite this

Nishimori, I., Bratanova, T., Toshkov, I., Caffrey, T., Mogaki, M., Shibata, Y., & Hollingsworth, M. A. (1995). Induction of experimental autoimmune sialoadenitis by immunization of PL/J mice with carbonic anhydrase II. Journal of Immunology, 154(9), 4865-4873.

Induction of experimental autoimmune sialoadenitis by immunization of PL/J mice with carbonic anhydrase II. / Nishimori, I.; Bratanova, T.; Toshkov, I.; Caffrey, T.; Mogaki, M.; Shibata, Y.; Hollingsworth, Michael A.

In: Journal of Immunology, Vol. 154, No. 9, 01.01.1995, p. 4865-4873.

Research output: Contribution to journalArticle

Nishimori, I, Bratanova, T, Toshkov, I, Caffrey, T, Mogaki, M, Shibata, Y & Hollingsworth, MA 1995, 'Induction of experimental autoimmune sialoadenitis by immunization of PL/J mice with carbonic anhydrase II', Journal of Immunology, vol. 154, no. 9, pp. 4865-4873.
Nishimori I, Bratanova T, Toshkov I, Caffrey T, Mogaki M, Shibata Y et al. Induction of experimental autoimmune sialoadenitis by immunization of PL/J mice with carbonic anhydrase II. Journal of Immunology. 1995 Jan 1;154(9):4865-4873.
Nishimori, I. ; Bratanova, T. ; Toshkov, I. ; Caffrey, T. ; Mogaki, M. ; Shibata, Y. ; Hollingsworth, Michael A. / Induction of experimental autoimmune sialoadenitis by immunization of PL/J mice with carbonic anhydrase II. In: Journal of Immunology. 1995 ; Vol. 154, No. 9. pp. 4865-4873.
@article{ab2654e313044d77a5bf1bab83226f7e,
title = "Induction of experimental autoimmune sialoadenitis by immunization of PL/J mice with carbonic anhydrase II",
abstract = "Experimental autoimmune sialoadenitis was induced in PL/J (H-2(u)) mice by intradermal immunization with human carbonic anhydrase II (CAII) and adjuvant containing monophosphoryl lipid A and trehalose diorynomycolate. Mice immunized with CAII showed a significant increase in the number and size of loci with lymphocytic infiltration in the salivary gland compared with mice immunized with adjuvant alone and untreated mice. In mice immunized with CAII, lymphocytic loci were observed around intercalated and intralobular ducts in the salivary glands, resulting in atrophy and replacement of acinar units. The epithelial cells of salivary ducts adjacent to the lymphocytic foci showed both degenerative and regenerative changes. Similar lymphocytic infiltrations were observed in the pancreas and kidney of a few mice immunized with CAII. Among several mouse strains with different H-2 haplotypes (p, q, r, s, and u), strains bearing H-2(s) and H-2(u) were susceptible to CAII-induced sialoadenitis. These results indicate that sialoadenitis induced by the immunization of CAII in mice may serve as a disease model of Sjogren's syndrome and that CAII or its derived peptides in association with the MHC may be one Ag recognized by an autoimmune response in this syndrome.",
author = "I. Nishimori and T. Bratanova and I. Toshkov and T. Caffrey and M. Mogaki and Y. Shibata and Hollingsworth, {Michael A}",
year = "1995",
month = "1",
day = "1",
language = "English (US)",
volume = "154",
pages = "4865--4873",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "9",

}

TY - JOUR

T1 - Induction of experimental autoimmune sialoadenitis by immunization of PL/J mice with carbonic anhydrase II

AU - Nishimori, I.

AU - Bratanova, T.

AU - Toshkov, I.

AU - Caffrey, T.

AU - Mogaki, M.

AU - Shibata, Y.

AU - Hollingsworth, Michael A

PY - 1995/1/1

Y1 - 1995/1/1

N2 - Experimental autoimmune sialoadenitis was induced in PL/J (H-2(u)) mice by intradermal immunization with human carbonic anhydrase II (CAII) and adjuvant containing monophosphoryl lipid A and trehalose diorynomycolate. Mice immunized with CAII showed a significant increase in the number and size of loci with lymphocytic infiltration in the salivary gland compared with mice immunized with adjuvant alone and untreated mice. In mice immunized with CAII, lymphocytic loci were observed around intercalated and intralobular ducts in the salivary glands, resulting in atrophy and replacement of acinar units. The epithelial cells of salivary ducts adjacent to the lymphocytic foci showed both degenerative and regenerative changes. Similar lymphocytic infiltrations were observed in the pancreas and kidney of a few mice immunized with CAII. Among several mouse strains with different H-2 haplotypes (p, q, r, s, and u), strains bearing H-2(s) and H-2(u) were susceptible to CAII-induced sialoadenitis. These results indicate that sialoadenitis induced by the immunization of CAII in mice may serve as a disease model of Sjogren's syndrome and that CAII or its derived peptides in association with the MHC may be one Ag recognized by an autoimmune response in this syndrome.

AB - Experimental autoimmune sialoadenitis was induced in PL/J (H-2(u)) mice by intradermal immunization with human carbonic anhydrase II (CAII) and adjuvant containing monophosphoryl lipid A and trehalose diorynomycolate. Mice immunized with CAII showed a significant increase in the number and size of loci with lymphocytic infiltration in the salivary gland compared with mice immunized with adjuvant alone and untreated mice. In mice immunized with CAII, lymphocytic loci were observed around intercalated and intralobular ducts in the salivary glands, resulting in atrophy and replacement of acinar units. The epithelial cells of salivary ducts adjacent to the lymphocytic foci showed both degenerative and regenerative changes. Similar lymphocytic infiltrations were observed in the pancreas and kidney of a few mice immunized with CAII. Among several mouse strains with different H-2 haplotypes (p, q, r, s, and u), strains bearing H-2(s) and H-2(u) were susceptible to CAII-induced sialoadenitis. These results indicate that sialoadenitis induced by the immunization of CAII in mice may serve as a disease model of Sjogren's syndrome and that CAII or its derived peptides in association with the MHC may be one Ag recognized by an autoimmune response in this syndrome.

UR - http://www.scopus.com/inward/record.url?scp=0029035240&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029035240&partnerID=8YFLogxK

M3 - Article

VL - 154

SP - 4865

EP - 4873

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 9

ER -