Induction of cyclooxygenase-2 by human monocytes exposed to group B streptococci

C. G. Maloney, S. D. Thompson, H. R. Hill, J. F. Bohnsack, T. M. McIntyre, G. A. Zimmerman

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Group B streptococcal (GBS) infections are associated with high morbidity and mortality. The molecular pathways mediating the pathophysiological events in GBS infection are not fully delineated. Cyclooxygenases (COX) are the enzymes that convert arachidonate to active eicosanoids. To identify the effects of GBS on eicosanoid metabolism and regulatory mechanisms, we exposed human monocytes to GBS and found that they secreted prostaglandin E2, prostacyclin, and thromboxane A2. Exposure to GBS caused monocytes to express COX-2 mRNA and protein in both a time- and concentration-dependent manner that correlated with eicosanoid production. COX-1 protein was unchanged. Addition of the anti-inflammatory cytokines interleukin (IL)-4 or IL-10 markedly attenuated GBS-induced COX-2 protein accumulation after GBS exposure, as did inhibition of p38 MAPK. Our experiments are the first to show that exposure of monocytes to a gram-positive bacterium (GBS) results in induction of functional COX-2, suggesting that eicosanoids may play important roles in the pathogenesis of GBS infections.

Original languageEnglish (US)
Pages (from-to)615-621
Number of pages7
JournalJournal of Leukocyte Biology
Volume67
Issue number5
DOIs
StatePublished - Jan 1 2000

Fingerprint

Streptococcus agalactiae
Eicosanoids
Cyclooxygenase 2
Streptococcal Infections
Monocytes
Cyclooxygenase 1
Thromboxane A2
Proteins
Gram-Positive Bacteria
p38 Mitogen-Activated Protein Kinases
Epoprostenol
Prostaglandin-Endoperoxide Synthases
Dinoprostone
Interleukin-4
Interleukin-10
Anti-Inflammatory Agents
Cytokines
Morbidity
Messenger RNA
Mortality

Keywords

  • Infectious immunity
  • Inflammatory mediators
  • Mononuclear phagocytes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Cell Biology

Cite this

Maloney, C. G., Thompson, S. D., Hill, H. R., Bohnsack, J. F., McIntyre, T. M., & Zimmerman, G. A. (2000). Induction of cyclooxygenase-2 by human monocytes exposed to group B streptococci. Journal of Leukocyte Biology, 67(5), 615-621. https://doi.org/10.1002/jlb.67.5.615

Induction of cyclooxygenase-2 by human monocytes exposed to group B streptococci. / Maloney, C. G.; Thompson, S. D.; Hill, H. R.; Bohnsack, J. F.; McIntyre, T. M.; Zimmerman, G. A.

In: Journal of Leukocyte Biology, Vol. 67, No. 5, 01.01.2000, p. 615-621.

Research output: Contribution to journalArticle

Maloney, CG, Thompson, SD, Hill, HR, Bohnsack, JF, McIntyre, TM & Zimmerman, GA 2000, 'Induction of cyclooxygenase-2 by human monocytes exposed to group B streptococci', Journal of Leukocyte Biology, vol. 67, no. 5, pp. 615-621. https://doi.org/10.1002/jlb.67.5.615
Maloney, C. G. ; Thompson, S. D. ; Hill, H. R. ; Bohnsack, J. F. ; McIntyre, T. M. ; Zimmerman, G. A. / Induction of cyclooxygenase-2 by human monocytes exposed to group B streptococci. In: Journal of Leukocyte Biology. 2000 ; Vol. 67, No. 5. pp. 615-621.
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