Induction of colonic aberrant crypts in mice by feeding apparent N-nitroso compounds derived from hot dogs.

Michael E. Davis, Michal P. Lisowyj, Lin Zhou, James L. Wisecarver, James M. Gulizia, Valerie K. Shostrom, Nathalie Naud, Denis E. Corpet, Sidney S. Mirvish

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Nitrite-preserved meats (e.g., hot dogs) may help cause colon cancer because they contain N-nitroso compounds. We tested whether purified hot-dog-derived total apparent N-nitroso compounds (ANC) could induce colonic aberrant crypts, which are putative precursors of colon cancer. We purified ANC precursors in hot dogs and nitrosated them to produce ANC. In preliminary tests, CF1 mice received 1 or 3 i.p. injections of 5 mg azoxymethane (AOM)/kg. In Experiments 1 and 2, female A/J mice received ANC in diet. In Experiment 1, ANC dose initially dropped sharply because the ANC precursors had mostly decomposed but, later in Experiment 1 and throughout Experiment 2, ANC remained at 85 nmol/g diet. Mice were killed after 8 (AOM tests) or 17-34 (ANC tests) wk. Median numbers of aberrant crypts in the distal 2 cm of the colon for 1 and 3 AOM injections, CF1 controls, ANC (Experiment 1), ANC (Experiment 2),and untreated A/J mice were 31, 74, 12, 20, 12, and 5-6, with P < 0.01 for both ANC tests. Experiment 2 showed somewhat increased numbers of colonic mucin-depleted foci in the ANC-treated group. We conclude that hot-dog-derived ANC induced significant numbers of aberrant crypts in the mouse colon.

Original languageEnglish (US)
Pages (from-to)342-349
Number of pages8
JournalNutrition and cancer
Volume64
Issue number2
DOIs
StatePublished - 2012

Fingerprint

Nitroso Compounds
Dogs
Azoxymethane
Colonic Neoplasms
Colon
Diet
Injections
Mucins
Nitrites

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Oncology
  • Nutrition and Dietetics
  • Cancer Research

Cite this

Induction of colonic aberrant crypts in mice by feeding apparent N-nitroso compounds derived from hot dogs. / Davis, Michael E.; Lisowyj, Michal P.; Zhou, Lin; Wisecarver, James L.; Gulizia, James M.; Shostrom, Valerie K.; Naud, Nathalie; Corpet, Denis E.; Mirvish, Sidney S.

In: Nutrition and cancer, Vol. 64, No. 2, 2012, p. 342-349.

Research output: Contribution to journalArticle

Davis, ME, Lisowyj, MP, Zhou, L, Wisecarver, JL, Gulizia, JM, Shostrom, VK, Naud, N, Corpet, DE & Mirvish, SS 2012, 'Induction of colonic aberrant crypts in mice by feeding apparent N-nitroso compounds derived from hot dogs.', Nutrition and cancer, vol. 64, no. 2, pp. 342-349. https://doi.org/10.1080/01635581.2012.650777
Davis, Michael E. ; Lisowyj, Michal P. ; Zhou, Lin ; Wisecarver, James L. ; Gulizia, James M. ; Shostrom, Valerie K. ; Naud, Nathalie ; Corpet, Denis E. ; Mirvish, Sidney S. / Induction of colonic aberrant crypts in mice by feeding apparent N-nitroso compounds derived from hot dogs. In: Nutrition and cancer. 2012 ; Vol. 64, No. 2. pp. 342-349.
@article{fda4eb012b57446085d965f7916838f8,
title = "Induction of colonic aberrant crypts in mice by feeding apparent N-nitroso compounds derived from hot dogs.",
abstract = "Nitrite-preserved meats (e.g., hot dogs) may help cause colon cancer because they contain N-nitroso compounds. We tested whether purified hot-dog-derived total apparent N-nitroso compounds (ANC) could induce colonic aberrant crypts, which are putative precursors of colon cancer. We purified ANC precursors in hot dogs and nitrosated them to produce ANC. In preliminary tests, CF1 mice received 1 or 3 i.p. injections of 5 mg azoxymethane (AOM)/kg. In Experiments 1 and 2, female A/J mice received ANC in diet. In Experiment 1, ANC dose initially dropped sharply because the ANC precursors had mostly decomposed but, later in Experiment 1 and throughout Experiment 2, ANC remained at 85 nmol/g diet. Mice were killed after 8 (AOM tests) or 17-34 (ANC tests) wk. Median numbers of aberrant crypts in the distal 2 cm of the colon for 1 and 3 AOM injections, CF1 controls, ANC (Experiment 1), ANC (Experiment 2),and untreated A/J mice were 31, 74, 12, 20, 12, and 5-6, with P < 0.01 for both ANC tests. Experiment 2 showed somewhat increased numbers of colonic mucin-depleted foci in the ANC-treated group. We conclude that hot-dog-derived ANC induced significant numbers of aberrant crypts in the mouse colon.",
author = "Davis, {Michael E.} and Lisowyj, {Michal P.} and Lin Zhou and Wisecarver, {James L.} and Gulizia, {James M.} and Shostrom, {Valerie K.} and Nathalie Naud and Corpet, {Denis E.} and Mirvish, {Sidney S.}",
year = "2012",
doi = "10.1080/01635581.2012.650777",
language = "English (US)",
volume = "64",
pages = "342--349",
journal = "Nutrition and Cancer",
issn = "0163-5581",
publisher = "Routledge",
number = "2",

}

TY - JOUR

T1 - Induction of colonic aberrant crypts in mice by feeding apparent N-nitroso compounds derived from hot dogs.

AU - Davis, Michael E.

AU - Lisowyj, Michal P.

AU - Zhou, Lin

AU - Wisecarver, James L.

AU - Gulizia, James M.

AU - Shostrom, Valerie K.

AU - Naud, Nathalie

AU - Corpet, Denis E.

AU - Mirvish, Sidney S.

PY - 2012

Y1 - 2012

N2 - Nitrite-preserved meats (e.g., hot dogs) may help cause colon cancer because they contain N-nitroso compounds. We tested whether purified hot-dog-derived total apparent N-nitroso compounds (ANC) could induce colonic aberrant crypts, which are putative precursors of colon cancer. We purified ANC precursors in hot dogs and nitrosated them to produce ANC. In preliminary tests, CF1 mice received 1 or 3 i.p. injections of 5 mg azoxymethane (AOM)/kg. In Experiments 1 and 2, female A/J mice received ANC in diet. In Experiment 1, ANC dose initially dropped sharply because the ANC precursors had mostly decomposed but, later in Experiment 1 and throughout Experiment 2, ANC remained at 85 nmol/g diet. Mice were killed after 8 (AOM tests) or 17-34 (ANC tests) wk. Median numbers of aberrant crypts in the distal 2 cm of the colon for 1 and 3 AOM injections, CF1 controls, ANC (Experiment 1), ANC (Experiment 2),and untreated A/J mice were 31, 74, 12, 20, 12, and 5-6, with P < 0.01 for both ANC tests. Experiment 2 showed somewhat increased numbers of colonic mucin-depleted foci in the ANC-treated group. We conclude that hot-dog-derived ANC induced significant numbers of aberrant crypts in the mouse colon.

AB - Nitrite-preserved meats (e.g., hot dogs) may help cause colon cancer because they contain N-nitroso compounds. We tested whether purified hot-dog-derived total apparent N-nitroso compounds (ANC) could induce colonic aberrant crypts, which are putative precursors of colon cancer. We purified ANC precursors in hot dogs and nitrosated them to produce ANC. In preliminary tests, CF1 mice received 1 or 3 i.p. injections of 5 mg azoxymethane (AOM)/kg. In Experiments 1 and 2, female A/J mice received ANC in diet. In Experiment 1, ANC dose initially dropped sharply because the ANC precursors had mostly decomposed but, later in Experiment 1 and throughout Experiment 2, ANC remained at 85 nmol/g diet. Mice were killed after 8 (AOM tests) or 17-34 (ANC tests) wk. Median numbers of aberrant crypts in the distal 2 cm of the colon for 1 and 3 AOM injections, CF1 controls, ANC (Experiment 1), ANC (Experiment 2),and untreated A/J mice were 31, 74, 12, 20, 12, and 5-6, with P < 0.01 for both ANC tests. Experiment 2 showed somewhat increased numbers of colonic mucin-depleted foci in the ANC-treated group. We conclude that hot-dog-derived ANC induced significant numbers of aberrant crypts in the mouse colon.

UR - http://www.scopus.com/inward/record.url?scp=84862257696&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84862257696&partnerID=8YFLogxK

U2 - 10.1080/01635581.2012.650777

DO - 10.1080/01635581.2012.650777

M3 - Article

C2 - 22293095

AN - SCOPUS:84862257696

VL - 64

SP - 342

EP - 349

JO - Nutrition and Cancer

JF - Nutrition and Cancer

SN - 0163-5581

IS - 2

ER -