Induction of calcyclin after ischemic injury to rat kidney

Andrew J.P. Lewington, Babu J. Padanilam, Marc R. Hammerman

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Genes differentially expressed after acute renal ischemic injury were identified using differential display-polymerase chain reaction (DD-PCR). Messenger RNA for calcyclin, a member of the S100 family of calcium-binding proteins, is increased in kidneys by 6 h following ischemic injury to rats compared with sham surgery. The level of calcyclin mRNA is increased 10-fold by 1 day postinjury and declines thereafter. In situ hybridization demonstrates little calcyclin mRNA in kidneys of sham-operated rats. However, calcyclin protein is present in glomeruli and distal tubules (DT). Compared with kidneys from sham-operated controls, both calcyclin mRNA and protein expression are increased at 1-3 days following ischemic injury in the thick ascending limb of Henle the DT, and in damaged regenerating segments of proximal tubules. By 7 days postischemia there is a reduction in mRNA and protein expression. Calcyclin could play a role in the regulation of renal cell proliferation and regeneration in the recovery process after acute ischemic injury.

Original languageEnglish (US)
Pages (from-to)F380-F385
JournalAmerican Journal of Physiology - Renal Physiology
Volume273
Issue number3 42-3
StatePublished - Sep 1 1997

Fingerprint

Kidney
Messenger RNA
Wounds and Injuries
Proteins
Calcium-Binding Proteins
Acute Kidney Injury
In Situ Hybridization
Regeneration
Extremities
Cell Proliferation
Polymerase Chain Reaction
Genes

Keywords

  • Acute renal failure
  • Differential display
  • Polymerase-chain reaction

ASJC Scopus subject areas

  • Physiology
  • Urology

Cite this

Induction of calcyclin after ischemic injury to rat kidney. / Lewington, Andrew J.P.; Padanilam, Babu J.; Hammerman, Marc R.

In: American Journal of Physiology - Renal Physiology, Vol. 273, No. 3 42-3, 01.09.1997, p. F380-F385.

Research output: Contribution to journalArticle

Lewington, Andrew J.P. ; Padanilam, Babu J. ; Hammerman, Marc R. / Induction of calcyclin after ischemic injury to rat kidney. In: American Journal of Physiology - Renal Physiology. 1997 ; Vol. 273, No. 3 42-3. pp. F380-F385.
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