Inducible resistance to oxidant stress in the protozoan Leishmania chagasi

Melissa A. Miller, Stephen E. McGowan, Kira R. Gantt, Matthew Champion, Sherry L. Novick, Kurt A. Andersen, Cyrus J. Bacchi, Nigel Yarlett, Bradley E. Britigan, Mary E. Wilson

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

Leishmania sp. protozoa are introduced into a mammalian skin by a sandfly vector, whereupon they encounter increased temperature and toxic oxidants generated during phagocytosis. We studied the effects of 37 °C 'heat shock' or sublethal menadione, which generates superoxide and hydrogen peroxide, on Leishmania chagasi virulence. Both heat and menadione caused parasites to become more resistant to H2O2-mediated toxicity. Peroxide resistance was also induced as promastigotes developed in culture from logarithmic to their virulent stationary phase form. Peroxide resistance was not associated with an increase in reduced thiols (trypanothione and glutathione) or increased activity of ornithine decarboxylase, which is rate-limiting in trypanothione synthesis. Membrane lipophosphoglycan increased in size as parasites developed to stationary phase but not after environmental exposures. Instead, parasites underwent a heat shock response upon exposure to heat or sublethal menadione, detected by increased levels of HSP70. Transfection of promastigotes with L. chagasi HSP70 caused a heat-inducible increase in resistance to peroxide, implying it is involved in antioxidant defense. We conclude that leishmania have redundant mechanisms for resisting toxic oxidants. Some are induced during developmental change and others are induced in response to environmental stress.

Original languageEnglish (US)
Pages (from-to)33883-33889
Number of pages7
JournalJournal of Biological Chemistry
Volume275
Issue number43
DOIs
StatePublished - Oct 27 2000

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Leishmania infantum
Vitamin K 3
Oxidants
Peroxides
Hot Temperature
Parasites
Poisons
Leishmania
Heat-Shock Response
Psychodidae
Ornithine Decarboxylase
Protozoa
Environmental Exposure
Phagocytosis
Sulfhydryl Compounds
Superoxides
Hydrogen Peroxide
Transfection
Glutathione
Virulence

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Miller, M. A., McGowan, S. E., Gantt, K. R., Champion, M., Novick, S. L., Andersen, K. A., ... Wilson, M. E. (2000). Inducible resistance to oxidant stress in the protozoan Leishmania chagasi. Journal of Biological Chemistry, 275(43), 33883-33889. https://doi.org/10.1074/jbc.M003671200

Inducible resistance to oxidant stress in the protozoan Leishmania chagasi. / Miller, Melissa A.; McGowan, Stephen E.; Gantt, Kira R.; Champion, Matthew; Novick, Sherry L.; Andersen, Kurt A.; Bacchi, Cyrus J.; Yarlett, Nigel; Britigan, Bradley E.; Wilson, Mary E.

In: Journal of Biological Chemistry, Vol. 275, No. 43, 27.10.2000, p. 33883-33889.

Research output: Contribution to journalArticle

Miller, MA, McGowan, SE, Gantt, KR, Champion, M, Novick, SL, Andersen, KA, Bacchi, CJ, Yarlett, N, Britigan, BE & Wilson, ME 2000, 'Inducible resistance to oxidant stress in the protozoan Leishmania chagasi', Journal of Biological Chemistry, vol. 275, no. 43, pp. 33883-33889. https://doi.org/10.1074/jbc.M003671200
Miller MA, McGowan SE, Gantt KR, Champion M, Novick SL, Andersen KA et al. Inducible resistance to oxidant stress in the protozoan Leishmania chagasi. Journal of Biological Chemistry. 2000 Oct 27;275(43):33883-33889. https://doi.org/10.1074/jbc.M003671200
Miller, Melissa A. ; McGowan, Stephen E. ; Gantt, Kira R. ; Champion, Matthew ; Novick, Sherry L. ; Andersen, Kurt A. ; Bacchi, Cyrus J. ; Yarlett, Nigel ; Britigan, Bradley E. ; Wilson, Mary E. / Inducible resistance to oxidant stress in the protozoan Leishmania chagasi. In: Journal of Biological Chemistry. 2000 ; Vol. 275, No. 43. pp. 33883-33889.
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