Indomethacin reduces acute baroreceptor resetting in the dog.

W. Wang, M. Brandle, Irving H Zucker

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

1. The influence of local perfusion of the carotid sinus with the cyclo‐oxygenase blocker indomethacin on acute baroreceptor resetting was investigated in anaesthetized dogs. 2. The carotid sinus was isolated and perfused with oxygenated Krebs‐Henseleit solution. Single units from the carotid sinus nerve were recorded. The carotid sinus was conditioned with static pressures of 50, 100 and 150 mmHg for 15 min. Following the conditioning period a carotid sinus pressure (CSP)‐discharge curve was constructed using static stepwise increases in CSP. 3. Perfusion of the carotid sinus with indomethacin (20 microM) caused an elevation in the threshold at every conditioning pressure (96.8 +/‐ 4.8 vs. 83.8 +/‐ 4.1 mmHg for 100 mmHg, P < 0.01; 91.9 +/‐ 4.7 vs. 70.3 +/‐ 4.4 mmHg for 50 mmHg, P < 0.01; and 103.5 +/‐ 4.4 vs. 96.4 +/‐ 4.1 mmHg for 150 mmHg, P = 0.06, respectively) and a significant reduction in the peak discharge rate at each conditioning pressure (32.0 +/‐ 4.4 vs. 48.2 +/‐ 5.5 spikes/s for 100 mmHg, P < 0.01; 32.2 +/‐ 5.8 vs. 45.8 +/‐ 5.1 spikes/s for 50 mmHg, P < 0.01; 32.0 +/‐ 3.7 vs. 51.1 +/‐ 8.1 spikes/s for 150 mmHg, P < 0.01). The resetting ratio (change in threshold/change in conditioning CSP) for both downward resetting (conditioning CSP from 100 to 50 mmHg) and upward resetting (conditioning CSP from 100 to 150 mmHg) was significantly decreased after indomethacin (0.10 +/‐ 0.02 vs. 0.27 +/‐ 0.02 for downward resetting, P < 0.01 and 0.14 +/‐ 0.03 vs. 0.25 +/‐ 0.01 for upward resetting, P < 0.01, respectively). 4. Conversely, perfusion of the carotid sinus with arachidonic acid (20 microM) significantly decreased the threshold at each conditioning CSP and increased the peak discharge rate at each conditioning CSP. The resetting ratio was significantly increased after arachidonic acid. 5. There was no change in resting carotid diameter or in the carotid sinus pressure‐carotid diameter relationship after indomethacin or arachidonic acid. 6. Removal of the endothelial cells in the carotid area with saponin did not prevent the effects of indomethacin on baroreceptor resetting. 7. These results suggest that prostaglandins have a tonic effect on the activity of the carotid sinus baroreceptor and may play a role in acute baroreceptor resetting.

Original languageEnglish (US)
Pages (from-to)139-151
Number of pages13
JournalThe Journal of Physiology
Volume469
Issue number1
DOIs
StatePublished - Sep 1 1993

Fingerprint

Carotid Sinus
Pressoreceptors
Indomethacin
Dogs
Pressure
Arachidonic Acid
Perfusion
Saponins
Prostaglandin-Endoperoxide Synthases

ASJC Scopus subject areas

  • Physiology

Cite this

Indomethacin reduces acute baroreceptor resetting in the dog. / Wang, W.; Brandle, M.; Zucker, Irving H.

In: The Journal of Physiology, Vol. 469, No. 1, 01.09.1993, p. 139-151.

Research output: Contribution to journalArticle

@article{2b3576b3db6d49fa986dcfd4e9ba0dea,
title = "Indomethacin reduces acute baroreceptor resetting in the dog.",
abstract = "1. The influence of local perfusion of the carotid sinus with the cyclo‐oxygenase blocker indomethacin on acute baroreceptor resetting was investigated in anaesthetized dogs. 2. The carotid sinus was isolated and perfused with oxygenated Krebs‐Henseleit solution. Single units from the carotid sinus nerve were recorded. The carotid sinus was conditioned with static pressures of 50, 100 and 150 mmHg for 15 min. Following the conditioning period a carotid sinus pressure (CSP)‐discharge curve was constructed using static stepwise increases in CSP. 3. Perfusion of the carotid sinus with indomethacin (20 microM) caused an elevation in the threshold at every conditioning pressure (96.8 +/‐ 4.8 vs. 83.8 +/‐ 4.1 mmHg for 100 mmHg, P < 0.01; 91.9 +/‐ 4.7 vs. 70.3 +/‐ 4.4 mmHg for 50 mmHg, P < 0.01; and 103.5 +/‐ 4.4 vs. 96.4 +/‐ 4.1 mmHg for 150 mmHg, P = 0.06, respectively) and a significant reduction in the peak discharge rate at each conditioning pressure (32.0 +/‐ 4.4 vs. 48.2 +/‐ 5.5 spikes/s for 100 mmHg, P < 0.01; 32.2 +/‐ 5.8 vs. 45.8 +/‐ 5.1 spikes/s for 50 mmHg, P < 0.01; 32.0 +/‐ 3.7 vs. 51.1 +/‐ 8.1 spikes/s for 150 mmHg, P < 0.01). The resetting ratio (change in threshold/change in conditioning CSP) for both downward resetting (conditioning CSP from 100 to 50 mmHg) and upward resetting (conditioning CSP from 100 to 150 mmHg) was significantly decreased after indomethacin (0.10 +/‐ 0.02 vs. 0.27 +/‐ 0.02 for downward resetting, P < 0.01 and 0.14 +/‐ 0.03 vs. 0.25 +/‐ 0.01 for upward resetting, P < 0.01, respectively). 4. Conversely, perfusion of the carotid sinus with arachidonic acid (20 microM) significantly decreased the threshold at each conditioning CSP and increased the peak discharge rate at each conditioning CSP. The resetting ratio was significantly increased after arachidonic acid. 5. There was no change in resting carotid diameter or in the carotid sinus pressure‐carotid diameter relationship after indomethacin or arachidonic acid. 6. Removal of the endothelial cells in the carotid area with saponin did not prevent the effects of indomethacin on baroreceptor resetting. 7. These results suggest that prostaglandins have a tonic effect on the activity of the carotid sinus baroreceptor and may play a role in acute baroreceptor resetting.",
author = "W. Wang and M. Brandle and Zucker, {Irving H}",
year = "1993",
month = "9",
day = "1",
doi = "10.1113/jphysiol.1993.sp019808",
language = "English (US)",
volume = "469",
pages = "139--151",
journal = "Journal of Physiology",
issn = "0022-3751",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Indomethacin reduces acute baroreceptor resetting in the dog.

AU - Wang, W.

AU - Brandle, M.

AU - Zucker, Irving H

PY - 1993/9/1

Y1 - 1993/9/1

N2 - 1. The influence of local perfusion of the carotid sinus with the cyclo‐oxygenase blocker indomethacin on acute baroreceptor resetting was investigated in anaesthetized dogs. 2. The carotid sinus was isolated and perfused with oxygenated Krebs‐Henseleit solution. Single units from the carotid sinus nerve were recorded. The carotid sinus was conditioned with static pressures of 50, 100 and 150 mmHg for 15 min. Following the conditioning period a carotid sinus pressure (CSP)‐discharge curve was constructed using static stepwise increases in CSP. 3. Perfusion of the carotid sinus with indomethacin (20 microM) caused an elevation in the threshold at every conditioning pressure (96.8 +/‐ 4.8 vs. 83.8 +/‐ 4.1 mmHg for 100 mmHg, P < 0.01; 91.9 +/‐ 4.7 vs. 70.3 +/‐ 4.4 mmHg for 50 mmHg, P < 0.01; and 103.5 +/‐ 4.4 vs. 96.4 +/‐ 4.1 mmHg for 150 mmHg, P = 0.06, respectively) and a significant reduction in the peak discharge rate at each conditioning pressure (32.0 +/‐ 4.4 vs. 48.2 +/‐ 5.5 spikes/s for 100 mmHg, P < 0.01; 32.2 +/‐ 5.8 vs. 45.8 +/‐ 5.1 spikes/s for 50 mmHg, P < 0.01; 32.0 +/‐ 3.7 vs. 51.1 +/‐ 8.1 spikes/s for 150 mmHg, P < 0.01). The resetting ratio (change in threshold/change in conditioning CSP) for both downward resetting (conditioning CSP from 100 to 50 mmHg) and upward resetting (conditioning CSP from 100 to 150 mmHg) was significantly decreased after indomethacin (0.10 +/‐ 0.02 vs. 0.27 +/‐ 0.02 for downward resetting, P < 0.01 and 0.14 +/‐ 0.03 vs. 0.25 +/‐ 0.01 for upward resetting, P < 0.01, respectively). 4. Conversely, perfusion of the carotid sinus with arachidonic acid (20 microM) significantly decreased the threshold at each conditioning CSP and increased the peak discharge rate at each conditioning CSP. The resetting ratio was significantly increased after arachidonic acid. 5. There was no change in resting carotid diameter or in the carotid sinus pressure‐carotid diameter relationship after indomethacin or arachidonic acid. 6. Removal of the endothelial cells in the carotid area with saponin did not prevent the effects of indomethacin on baroreceptor resetting. 7. These results suggest that prostaglandins have a tonic effect on the activity of the carotid sinus baroreceptor and may play a role in acute baroreceptor resetting.

AB - 1. The influence of local perfusion of the carotid sinus with the cyclo‐oxygenase blocker indomethacin on acute baroreceptor resetting was investigated in anaesthetized dogs. 2. The carotid sinus was isolated and perfused with oxygenated Krebs‐Henseleit solution. Single units from the carotid sinus nerve were recorded. The carotid sinus was conditioned with static pressures of 50, 100 and 150 mmHg for 15 min. Following the conditioning period a carotid sinus pressure (CSP)‐discharge curve was constructed using static stepwise increases in CSP. 3. Perfusion of the carotid sinus with indomethacin (20 microM) caused an elevation in the threshold at every conditioning pressure (96.8 +/‐ 4.8 vs. 83.8 +/‐ 4.1 mmHg for 100 mmHg, P < 0.01; 91.9 +/‐ 4.7 vs. 70.3 +/‐ 4.4 mmHg for 50 mmHg, P < 0.01; and 103.5 +/‐ 4.4 vs. 96.4 +/‐ 4.1 mmHg for 150 mmHg, P = 0.06, respectively) and a significant reduction in the peak discharge rate at each conditioning pressure (32.0 +/‐ 4.4 vs. 48.2 +/‐ 5.5 spikes/s for 100 mmHg, P < 0.01; 32.2 +/‐ 5.8 vs. 45.8 +/‐ 5.1 spikes/s for 50 mmHg, P < 0.01; 32.0 +/‐ 3.7 vs. 51.1 +/‐ 8.1 spikes/s for 150 mmHg, P < 0.01). The resetting ratio (change in threshold/change in conditioning CSP) for both downward resetting (conditioning CSP from 100 to 50 mmHg) and upward resetting (conditioning CSP from 100 to 150 mmHg) was significantly decreased after indomethacin (0.10 +/‐ 0.02 vs. 0.27 +/‐ 0.02 for downward resetting, P < 0.01 and 0.14 +/‐ 0.03 vs. 0.25 +/‐ 0.01 for upward resetting, P < 0.01, respectively). 4. Conversely, perfusion of the carotid sinus with arachidonic acid (20 microM) significantly decreased the threshold at each conditioning CSP and increased the peak discharge rate at each conditioning CSP. The resetting ratio was significantly increased after arachidonic acid. 5. There was no change in resting carotid diameter or in the carotid sinus pressure‐carotid diameter relationship after indomethacin or arachidonic acid. 6. Removal of the endothelial cells in the carotid area with saponin did not prevent the effects of indomethacin on baroreceptor resetting. 7. These results suggest that prostaglandins have a tonic effect on the activity of the carotid sinus baroreceptor and may play a role in acute baroreceptor resetting.

UR - http://www.scopus.com/inward/record.url?scp=0027275714&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027275714&partnerID=8YFLogxK

U2 - 10.1113/jphysiol.1993.sp019808

DO - 10.1113/jphysiol.1993.sp019808

M3 - Article

C2 - 8271195

AN - SCOPUS:0027275714

VL - 469

SP - 139

EP - 151

JO - Journal of Physiology

JF - Journal of Physiology

SN - 0022-3751

IS - 1

ER -