Individuality in gut microbiota composition is a complex polygenic trait shaped by multiple environmental and host genetic factors

Andrew K Benson, Scott A. Kelly, Ryan Legge, Fangrui Ma, Soo Jen Low, Jaehyoung Kim, Min Zhang, Phaik Lyn Oh, Derrick Nehrenberg, Kunjie Hua, Stephen D Kachman, Etsuko Moriyama, Jens Walter, Daniel A. Peterson, Daniel Pomp

Research output: Contribution to journalArticle

601 Citations (Scopus)

Abstract

In vertebrates, including humans, individuals harbor gut microbial communities whose species composition and relative proportions of dominant microbial groups are tremendously varied. Although external and stochastic factors clearly contribute to the individuality of the microbiota, the fundamental principles dictating how environmental factors and host genetic factors combine to shape this complex ecosystem are largely unknown and require systematic study. Here we examined factors that affect microbiota composition in a large (n = 645) mouse advanced intercross line originating from a cross between C57BL/6J and an ICR-derived outbred line (HR). Quantitative pyrosequencing of the microbiota defined a core measurable microbiota (CMM) of 64 conserved taxonomic groups that varied quantitatively across most animals in the population. Although some of this variation can be explained by litter and cohort effects, individual host genotype had a measurable contribution. Testing of the CMM abundances for cosegregation with 530 fully informative SNP markers identified 18 host quantitative trait loci (QTL) that show significant or suggestive genomewide linkage with relative abundances of specific microbial taxa. These QTL affect microbiota composition in three ways; some loci control individual microbial species, some control groups of related taxa, and some have putative pleiotropic effects on groups of distantly related organisms. These data provide clear evidence for the importance of host genetic control in shaping individual microbiome diversity in mammals, a key step toward understanding the factors that govern the assemblages of gut microbiota associated with complex diseases.

Original languageEnglish (US)
Pages (from-to)18933-18938
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume107
Issue number44
DOIs
StatePublished - Nov 2 2010

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Multifactorial Inheritance
Microbiota
Individuality
Quantitative Trait Loci
Cohort Effect
Internal-External Control
Gastrointestinal Microbiome
Single Nucleotide Polymorphism
Ecosystem
Vertebrates
Mammals
Genotype
Control Groups

Keywords

  • 16S rDNA
  • Microbiome phenotyping
  • Population
  • Pyrosequencing
  • Quantitative trait loci mapping

ASJC Scopus subject areas

  • General

Cite this

Individuality in gut microbiota composition is a complex polygenic trait shaped by multiple environmental and host genetic factors. / Benson, Andrew K; Kelly, Scott A.; Legge, Ryan; Ma, Fangrui; Low, Soo Jen; Kim, Jaehyoung; Zhang, Min; Oh, Phaik Lyn; Nehrenberg, Derrick; Hua, Kunjie; Kachman, Stephen D; Moriyama, Etsuko; Walter, Jens; Peterson, Daniel A.; Pomp, Daniel.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, No. 44, 02.11.2010, p. 18933-18938.

Research output: Contribution to journalArticle

Benson, Andrew K ; Kelly, Scott A. ; Legge, Ryan ; Ma, Fangrui ; Low, Soo Jen ; Kim, Jaehyoung ; Zhang, Min ; Oh, Phaik Lyn ; Nehrenberg, Derrick ; Hua, Kunjie ; Kachman, Stephen D ; Moriyama, Etsuko ; Walter, Jens ; Peterson, Daniel A. ; Pomp, Daniel. / Individuality in gut microbiota composition is a complex polygenic trait shaped by multiple environmental and host genetic factors. In: Proceedings of the National Academy of Sciences of the United States of America. 2010 ; Vol. 107, No. 44. pp. 18933-18938.
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