Indirect solid-phase microradioimmunoassay for detection of pseudorabies virus antibody in swine sera.

C. L. Kelling, W. L. Staudinger, M. B. Rhodes

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

An indirect solid-phase microradioimmunoassay (IRIA) was developed for detection and quantitation of antibodies to pseudorabies virus (PRV) in swine serum. Qualitative results of the IRIA compared closely with results of the serum neutralization test (NT) and the microimmunodiffusion test (MIDT). The IRIA was more sensitive than the NT for detection of antibodies to PRV in swine serum. The IRIA result is expressed numerically. With the IRIA and NT, antibody to PRV was first detectable in 3 experimentally infected pigs at 9 days after inoculation. With MIDT, antibody was detected in the 3 experimentally infected pigs at 9 days after inoculation. With the MIDT, antibody was detected in the 3 experimentally infected pigs at 7, 8, and 9 days after inoculation. The IRIA results are obtainable within a few hours; the NT and MIDT require 48 hours for completion.

Original languageEnglish (US)
Pages (from-to)1955-1957
Number of pages3
JournalAmerican journal of veterinary research
Volume39
Issue number12
StatePublished - Dec 1 1978

    Fingerprint

ASJC Scopus subject areas

  • veterinary(all)

Cite this