Independent variations of myc amplification, inducibility, maturation, and proliferation states in HL60

Gábor Szabó, Chris Bunce, Geoffrey Brown, George Klein, Janos Sumegi

Research output: Contribution to journalArticle

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Abstract

We have investigated the possible relationship between c-myc gene activity and other variable traits in HL60. In a panel of variant lines, a good correlation was observed between myc gene copy number and the level of myc mRNA. There was no correlation between myc amplification or expression and the resistance of the lines to induction of terminal neutrophilic or monocytic differentiation. Therefore, myc mRNA level does not appear to determine the ability of the variant HL60 lines to respond to inducers of differentiation. Flow cytometric analyses of the expression of a differentiation antigen (AGF 4.36) revealed stable negative and positive subpopulations in growing HL60. myc amplification, expression, and inducibility were identical in these subpopulations, suggesting that variation of these traits in HL60 sublines and variants is not due to maturation state differences. myc gene copy number was also identical in transferrin receptor positive (proliferating) and negative (resting) populations. These data contradict the notion that myc amplification has been important in determining the in vitro biological properties of HL60.

Original languageEnglish (US)
Pages (from-to)334-343
Number of pages10
JournalExperimental Cell Research
Volume175
Issue number2
DOIs
StatePublished - Jan 1 1988

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myc Genes
Gene Dosage
Messenger RNA
Transferrin Receptors
Differentiation Antigens
Population

ASJC Scopus subject areas

  • Cell Biology

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Independent variations of myc amplification, inducibility, maturation, and proliferation states in HL60. / Szabó, Gábor; Bunce, Chris; Brown, Geoffrey; Klein, George; Sumegi, Janos.

In: Experimental Cell Research, Vol. 175, No. 2, 01.01.1988, p. 334-343.

Research output: Contribution to journalArticle

Szabó, Gábor ; Bunce, Chris ; Brown, Geoffrey ; Klein, George ; Sumegi, Janos. / Independent variations of myc amplification, inducibility, maturation, and proliferation states in HL60. In: Experimental Cell Research. 1988 ; Vol. 175, No. 2. pp. 334-343.
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