Values of K(I) for nine proline analogs as inhibitors of proline chemotaxis and of proline transport were determined. Two of them inhibited transport at substantially lower concentrations than chemotaxis; two at substantially higher concentrations. Moreover, mutants, believed to be in the component that binds proline, were isolated that showed a shift of K(M) for transport to higher concentrations, one as much as 40-fold. However, chemotaxis was virtually unaffected. Therefore, unlike galactose chemotaxis and transport in Escherichia coli, which share the galactose-binding protein proline chemotaxis and transport in B. subtilis are independent.
|Original language||English (US)|
|Number of pages||4|
|Journal||Journal of Biological Chemistry|
|State||Published - Dec 1 1978|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology