Independence of proline chemotaxis and transport in Bacillus subtilis

G. W. Ordal, D. P. Villani, R. A. Nicholas, Frederick G Hamel

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13 Citations (Scopus)

Abstract

Values of K(I) for nine proline analogs as inhibitors of proline chemotaxis and of proline transport were determined. Two of them inhibited transport at substantially lower concentrations than chemotaxis; two at substantially higher concentrations. Moreover, mutants, believed to be in the component that binds proline, were isolated that showed a shift of K(M) for transport to higher concentrations, one as much as 40-fold. However, chemotaxis was virtually unaffected. Therefore, unlike galactose chemotaxis and transport in Escherichia coli, which share the galactose-binding protein proline chemotaxis and transport in B. subtilis are independent.

Original languageEnglish (US)
Pages (from-to)4916-4919
Number of pages4
JournalJournal of Biological Chemistry
Volume253
Issue number14
StatePublished - Dec 1 1978

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Bacilli
Chemotaxis
Bacillus subtilis
Proline
Galactose
Escherichia coli

ASJC Scopus subject areas

  • Biochemistry

Cite this

Ordal, G. W., Villani, D. P., Nicholas, R. A., & Hamel, F. G. (1978). Independence of proline chemotaxis and transport in Bacillus subtilis. Journal of Biological Chemistry, 253(14), 4916-4919.

Independence of proline chemotaxis and transport in Bacillus subtilis. / Ordal, G. W.; Villani, D. P.; Nicholas, R. A.; Hamel, Frederick G.

In: Journal of Biological Chemistry, Vol. 253, No. 14, 01.12.1978, p. 4916-4919.

Research output: Contribution to journalArticle

Ordal, GW, Villani, DP, Nicholas, RA & Hamel, FG 1978, 'Independence of proline chemotaxis and transport in Bacillus subtilis', Journal of Biological Chemistry, vol. 253, no. 14, pp. 4916-4919.
Ordal, G. W. ; Villani, D. P. ; Nicholas, R. A. ; Hamel, Frederick G. / Independence of proline chemotaxis and transport in Bacillus subtilis. In: Journal of Biological Chemistry. 1978 ; Vol. 253, No. 14. pp. 4916-4919.
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