Increasing the chemical potential of the germ-line antibody repertoire

Nora Sarvetnick, Deepak Gurushanthaiah, Nianhe Han, James Prudent, Peter Schultz, Richard Lerner

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

To augment the chemical potential of the immunological repertoire, a metal ion-binding light chain has been introduced into the murine genome. Mice containing the transgene were subsequently immunized with a fluorescein conjugate. The transgenic light chain was found at a high frequency in the anti-fluorescein memory B-cell compartment. This general method should be applicable to other cofactors and small molecules and should lead to generation of antibodies with unique catalytic activities.

Original languageEnglish (US)
Pages (from-to)4008-4011
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number9
DOIs
StatePublished - May 1 1993

Fingerprint

Fluorescein
Germ Cells
Light
Antibodies
Transgenes
B-Lymphocytes
Metals
Genome
Ions

Keywords

  • Catalysis
  • Cofactors
  • Coordination complex
  • Transgenic mice
  • Transition metals

ASJC Scopus subject areas

  • General

Cite this

Increasing the chemical potential of the germ-line antibody repertoire. / Sarvetnick, Nora; Gurushanthaiah, Deepak; Han, Nianhe; Prudent, James; Schultz, Peter; Lerner, Richard.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, No. 9, 01.05.1993, p. 4008-4011.

Research output: Contribution to journalArticle

Sarvetnick, Nora ; Gurushanthaiah, Deepak ; Han, Nianhe ; Prudent, James ; Schultz, Peter ; Lerner, Richard. / Increasing the chemical potential of the germ-line antibody repertoire. In: Proceedings of the National Academy of Sciences of the United States of America. 1993 ; Vol. 90, No. 9. pp. 4008-4011.
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