Increases in the concentration of brain α-bungarotoxin binding sites induced by dietary choline are age-dependent

Barbara J Morley, Laura L. Garner

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We have previously reported that a diet supplemented with choline induces an increase in the concentration of a brain nicotinic-like receptor, as measured by α-bungarotoxin (BuTX) binding. Here we report the effects of choline administered in the drinking water on BuTX binding in the cortex, midbrain and brainstem of rats at 3 ages. In comparison with animals fed a choline-free diet, choline supplementation produced increases averaging 50% in 23-day-old rats and increases of approximately 30% in 60-day-old rats. Increases were also found in 6-month-old animals (averaging 16%), but the differences were generally not statistically significant. The mechanism responsible for the increase in the concentration of BuTX binding sites following the administration of dietary choline is not known, but the results are discussed in terms of choline as a precursor for the biosynthesis of acetylcholine and the biosynthesis of phospholipids. These data indicate that the administration of dietary choline is not likely to be effective in reversing cholinergic deficits by increasing the concentration of nicotinic-like receptors in aging rats.

Original languageEnglish (US)
Pages (from-to)315-319
Number of pages5
JournalBrain Research
Volume378
Issue number2
DOIs
StatePublished - Jul 23 1986

Fingerprint

Bungarotoxins
Choline
Binding Sites
Brain
Nicotinic Receptors
Diet
Mesencephalon
Drinking Water
Cholinergic Agents
Brain Stem
Acetylcholine
Phospholipids

Keywords

  • brain nicotinic receptor
  • bungarotoxin
  • choline
  • rat

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Increases in the concentration of brain α-bungarotoxin binding sites induced by dietary choline are age-dependent. / Morley, Barbara J; Garner, Laura L.

In: Brain Research, Vol. 378, No. 2, 23.07.1986, p. 315-319.

Research output: Contribution to journalArticle

@article{eb1e6693bb5841e892240382896b9e08,
title = "Increases in the concentration of brain α-bungarotoxin binding sites induced by dietary choline are age-dependent",
abstract = "We have previously reported that a diet supplemented with choline induces an increase in the concentration of a brain nicotinic-like receptor, as measured by α-bungarotoxin (BuTX) binding. Here we report the effects of choline administered in the drinking water on BuTX binding in the cortex, midbrain and brainstem of rats at 3 ages. In comparison with animals fed a choline-free diet, choline supplementation produced increases averaging 50{\%} in 23-day-old rats and increases of approximately 30{\%} in 60-day-old rats. Increases were also found in 6-month-old animals (averaging 16{\%}), but the differences were generally not statistically significant. The mechanism responsible for the increase in the concentration of BuTX binding sites following the administration of dietary choline is not known, but the results are discussed in terms of choline as a precursor for the biosynthesis of acetylcholine and the biosynthesis of phospholipids. These data indicate that the administration of dietary choline is not likely to be effective in reversing cholinergic deficits by increasing the concentration of nicotinic-like receptors in aging rats.",
keywords = "brain nicotinic receptor, bungarotoxin, choline, rat",
author = "Morley, {Barbara J} and Garner, {Laura L.}",
year = "1986",
month = "7",
day = "23",
doi = "10.1016/0006-8993(86)90934-0",
language = "English (US)",
volume = "378",
pages = "315--319",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Increases in the concentration of brain α-bungarotoxin binding sites induced by dietary choline are age-dependent

AU - Morley, Barbara J

AU - Garner, Laura L.

PY - 1986/7/23

Y1 - 1986/7/23

N2 - We have previously reported that a diet supplemented with choline induces an increase in the concentration of a brain nicotinic-like receptor, as measured by α-bungarotoxin (BuTX) binding. Here we report the effects of choline administered in the drinking water on BuTX binding in the cortex, midbrain and brainstem of rats at 3 ages. In comparison with animals fed a choline-free diet, choline supplementation produced increases averaging 50% in 23-day-old rats and increases of approximately 30% in 60-day-old rats. Increases were also found in 6-month-old animals (averaging 16%), but the differences were generally not statistically significant. The mechanism responsible for the increase in the concentration of BuTX binding sites following the administration of dietary choline is not known, but the results are discussed in terms of choline as a precursor for the biosynthesis of acetylcholine and the biosynthesis of phospholipids. These data indicate that the administration of dietary choline is not likely to be effective in reversing cholinergic deficits by increasing the concentration of nicotinic-like receptors in aging rats.

AB - We have previously reported that a diet supplemented with choline induces an increase in the concentration of a brain nicotinic-like receptor, as measured by α-bungarotoxin (BuTX) binding. Here we report the effects of choline administered in the drinking water on BuTX binding in the cortex, midbrain and brainstem of rats at 3 ages. In comparison with animals fed a choline-free diet, choline supplementation produced increases averaging 50% in 23-day-old rats and increases of approximately 30% in 60-day-old rats. Increases were also found in 6-month-old animals (averaging 16%), but the differences were generally not statistically significant. The mechanism responsible for the increase in the concentration of BuTX binding sites following the administration of dietary choline is not known, but the results are discussed in terms of choline as a precursor for the biosynthesis of acetylcholine and the biosynthesis of phospholipids. These data indicate that the administration of dietary choline is not likely to be effective in reversing cholinergic deficits by increasing the concentration of nicotinic-like receptors in aging rats.

KW - brain nicotinic receptor

KW - bungarotoxin

KW - choline

KW - rat

UR - http://www.scopus.com/inward/record.url?scp=0022536045&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022536045&partnerID=8YFLogxK

U2 - 10.1016/0006-8993(86)90934-0

DO - 10.1016/0006-8993(86)90934-0

M3 - Article

C2 - 3730881

AN - SCOPUS:0022536045

VL - 378

SP - 315

EP - 319

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 2

ER -