Increased septal 5-HIAA efflux in rats that do not develop learned helplessness after inescapable stress

Patrick J. Ronan, Mark Steciuk, Gerald L. Kramer, Martin Kram, Frederick Petty

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Learned helplessness is a behavioral deficit that can be induced by exposure to inescapable stress. Previous studies have implicated the lateral septum in mediating this phenomenon, and in this brain region, serotonin plays an important role in the development, maintenance, prevention, and reversal of learned helplessness behavior. Using the technique of in vivo microdialysis, we measured the efflux of serotonin (5-HT), dopamine (DA), and their respective metabolites, 5-hydroxyindoleacetic acid (5-HIAA) and 3,4- dihydroxyphenylacetic acid (DOPAC), from the lateral septum of rats that either developed or did not develop learned helplessness. During the microdialysis session all rats were subjected to restraint stress. Control groups included naive, home cage rats as well as tested control rats that were subjected to the identical handling, restraint, and shuttlebox testing as the rats that received inescapable shock. Overall, levels of 5-HIAA were significantly higher in non-helpless rats. There were no significant effects of restraint or differences in levels of 5-HT, DA, or DOPAC. We propose that this increase in 5-HIAA is indicative of an overall increase in serotonin metabolism in the lateral septum of rats that do not become helpless after inescapable stress. This increased serotonin metabolism in the lateral septum may protect the animal from adverse behavioral consequences of inescapable stress.

Original languageEnglish (US)
Pages (from-to)101-106
Number of pages6
JournalJournal of Neuroscience Research
Volume61
Issue number1
DOIs
StatePublished - Jul 1 2000

Fingerprint

Learned Helplessness
Hydroxyindoleacetic Acid
Serotonin
Microdialysis
Dopamine
3,4-Dihydroxyphenylacetic Acid
Shock
Maintenance
Control Groups
Acids
Brain

Keywords

  • 5-HIAA
  • Depression
  • Immobilization
  • Lateral septum
  • Learned helplessness
  • Microdialysis
  • Restraint
  • Serotonin

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Increased septal 5-HIAA efflux in rats that do not develop learned helplessness after inescapable stress. / Ronan, Patrick J.; Steciuk, Mark; Kramer, Gerald L.; Kram, Martin; Petty, Frederick.

In: Journal of Neuroscience Research, Vol. 61, No. 1, 01.07.2000, p. 101-106.

Research output: Contribution to journalArticle

Ronan, Patrick J. ; Steciuk, Mark ; Kramer, Gerald L. ; Kram, Martin ; Petty, Frederick. / Increased septal 5-HIAA efflux in rats that do not develop learned helplessness after inescapable stress. In: Journal of Neuroscience Research. 2000 ; Vol. 61, No. 1. pp. 101-106.
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