Increased perimeter red cell concentration in filter paper bloodspot samples is consistent with constant-load size exclusion chromatography occurring during application

Douglas F. Stickle, Neil J. Rawlinson, James D. Landmark

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: Filter paper bloodspot samples exhibit increased red cell concentration near the bloodspot perimeter compared to the interior. We examined whether simulation of size exclusion chromatography separating cell and liquid components during bloodspot formation was consistent with the observed red cell distribution. Methods: Whole blood was defined as a mixture of 60% liquid and 40% solids (hematocrit). Bloodspot formation was simulated by step-wise center additions of 1 μl blood up to a total volume of 50 μl. Partitioning of the liquid component of the liquid volume into space not available to the solid component was calculated using a partitioning coefficient K = Vi / Vm, where Vi is the immobile (stationary) liquid volume and Vm is the mobile liquid volume. After each volume addition step, relative red cell concentration was calculated as the solids volume fraction of total volume as a function of radial distance from the center. Results: Simulation for K = 0.3 resulted in final red cell distribution that was quantitatively consistent with bloodspot data. Conclusions: Increased perimeter red cell concentration in bloodspots is consistent with partitioning of the liquid fraction into mobile and immobile components during bloodspot formation according to the principles of constant-load size exclusion chromatography.

Original languageEnglish (US)
Pages (from-to)42-45
Number of pages4
JournalClinica Chimica Acta
Volume401
Issue number1-2
DOIs
StatePublished - Mar 1 2009

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Size exclusion chromatography
Gel Chromatography
Cells
Liquids
Blood
Cellular Structures
Hematocrit
Volume fraction

Keywords

  • Bloodspots
  • Filter paper
  • Preanalytical variables

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Increased perimeter red cell concentration in filter paper bloodspot samples is consistent with constant-load size exclusion chromatography occurring during application. / Stickle, Douglas F.; Rawlinson, Neil J.; Landmark, James D.

In: Clinica Chimica Acta, Vol. 401, No. 1-2, 01.03.2009, p. 42-45.

Research output: Contribution to journalArticle

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