Increased intestinal contents viscosity reduces cholesterol absorption efficiency in hamsters fed hydroxypropyl methylcellulose

Timothy P. Carr, Daniel D. Gallaher, Ching Hui Yang, Craig A. Hassel

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

This study was conducted to test the hypothesis that increased intestinal contents viscosity lowers plasma cholesterol concentrations by decreasing cholesterol absorption. Male Golden Syrian hamsters were fed for 4 wk diets containing 0.12% cholesterol, and either 4% cellulose or four different viscosity grades of hydroxypropyl methylcellulose (HPMC). Dietary HPMC confers viscosity in the small intestine but is resistant to fermentation. Cholesterol absorption efficiency was measured using the dual isotope ratio method, and plasma and liver cholesterol concentrations were determined enzymatically. Ex vivo viscosity of intestinal contents supernatants was measured using a Wells-Brookfield cone/plate viscometer, and the means of treatment groups ranged from 6 to 6532 mPa · s. Relative to dietary cellulose, all viscosity grades of HPMC resulted in significantly lower cholesterol absorption efficiency, lower plasma cholesterol concentration, and lower liver cholesteryl ester content. The logarithm of intestinal contents ex vivo viscosity was inversely correlated with dietary cholesterol absorption (r2 = 0.84, P = 0.028). Furthermore, dietary cholesterol absorption was positively correlated with plasma cholesterol concentration (r2 = 0.89, P = 0.017) and liver cholesteryl ester content (r2 = 0.96, P = 0.0031). Thus, the data suggest an independent role of intestinal contents viscosity in lowering plasma cholesterol concentration and liver cholesteryl ester content by reducing cholesterol absorption efficiency.

Original languageEnglish (US)
Pages (from-to)1463-1469
Number of pages7
JournalJournal of Nutrition
Volume126
Issue number5
StatePublished - May 1 1996

Fingerprint

Gastrointestinal Contents
methylcellulose
hamsters
Viscosity
Cricetinae
viscosity
Cholesterol
cholesterol
Cholesterol Esters
cholesteryl esters
Dietary Cholesterol
Liver
liver
Cellulose
Hypromellose Derivatives
cellulose
Mesocricetus
viscometers
Isotopes
Fermentation

Keywords

  • cholesterol absorption
  • dietary fiber
  • hamsters
  • plasma cholesterol
  • viscosity

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Increased intestinal contents viscosity reduces cholesterol absorption efficiency in hamsters fed hydroxypropyl methylcellulose. / Carr, Timothy P.; Gallaher, Daniel D.; Yang, Ching Hui; Hassel, Craig A.

In: Journal of Nutrition, Vol. 126, No. 5, 01.05.1996, p. 1463-1469.

Research output: Contribution to journalArticle

@article{4b56cffa99714234b805d8f4c76688d6,
title = "Increased intestinal contents viscosity reduces cholesterol absorption efficiency in hamsters fed hydroxypropyl methylcellulose",
abstract = "This study was conducted to test the hypothesis that increased intestinal contents viscosity lowers plasma cholesterol concentrations by decreasing cholesterol absorption. Male Golden Syrian hamsters were fed for 4 wk diets containing 0.12{\%} cholesterol, and either 4{\%} cellulose or four different viscosity grades of hydroxypropyl methylcellulose (HPMC). Dietary HPMC confers viscosity in the small intestine but is resistant to fermentation. Cholesterol absorption efficiency was measured using the dual isotope ratio method, and plasma and liver cholesterol concentrations were determined enzymatically. Ex vivo viscosity of intestinal contents supernatants was measured using a Wells-Brookfield cone/plate viscometer, and the means of treatment groups ranged from 6 to 6532 mPa · s. Relative to dietary cellulose, all viscosity grades of HPMC resulted in significantly lower cholesterol absorption efficiency, lower plasma cholesterol concentration, and lower liver cholesteryl ester content. The logarithm of intestinal contents ex vivo viscosity was inversely correlated with dietary cholesterol absorption (r2 = 0.84, P = 0.028). Furthermore, dietary cholesterol absorption was positively correlated with plasma cholesterol concentration (r2 = 0.89, P = 0.017) and liver cholesteryl ester content (r2 = 0.96, P = 0.0031). Thus, the data suggest an independent role of intestinal contents viscosity in lowering plasma cholesterol concentration and liver cholesteryl ester content by reducing cholesterol absorption efficiency.",
keywords = "cholesterol absorption, dietary fiber, hamsters, plasma cholesterol, viscosity",
author = "Carr, {Timothy P.} and Gallaher, {Daniel D.} and Yang, {Ching Hui} and Hassel, {Craig A.}",
year = "1996",
month = "5",
day = "1",
language = "English (US)",
volume = "126",
pages = "1463--1469",
journal = "The Journal of nutrition",
issn = "0022-3166",
publisher = "American Society for Nutrition",
number = "5",

}

TY - JOUR

T1 - Increased intestinal contents viscosity reduces cholesterol absorption efficiency in hamsters fed hydroxypropyl methylcellulose

AU - Carr, Timothy P.

AU - Gallaher, Daniel D.

AU - Yang, Ching Hui

AU - Hassel, Craig A.

PY - 1996/5/1

Y1 - 1996/5/1

N2 - This study was conducted to test the hypothesis that increased intestinal contents viscosity lowers plasma cholesterol concentrations by decreasing cholesterol absorption. Male Golden Syrian hamsters were fed for 4 wk diets containing 0.12% cholesterol, and either 4% cellulose or four different viscosity grades of hydroxypropyl methylcellulose (HPMC). Dietary HPMC confers viscosity in the small intestine but is resistant to fermentation. Cholesterol absorption efficiency was measured using the dual isotope ratio method, and plasma and liver cholesterol concentrations were determined enzymatically. Ex vivo viscosity of intestinal contents supernatants was measured using a Wells-Brookfield cone/plate viscometer, and the means of treatment groups ranged from 6 to 6532 mPa · s. Relative to dietary cellulose, all viscosity grades of HPMC resulted in significantly lower cholesterol absorption efficiency, lower plasma cholesterol concentration, and lower liver cholesteryl ester content. The logarithm of intestinal contents ex vivo viscosity was inversely correlated with dietary cholesterol absorption (r2 = 0.84, P = 0.028). Furthermore, dietary cholesterol absorption was positively correlated with plasma cholesterol concentration (r2 = 0.89, P = 0.017) and liver cholesteryl ester content (r2 = 0.96, P = 0.0031). Thus, the data suggest an independent role of intestinal contents viscosity in lowering plasma cholesterol concentration and liver cholesteryl ester content by reducing cholesterol absorption efficiency.

AB - This study was conducted to test the hypothesis that increased intestinal contents viscosity lowers plasma cholesterol concentrations by decreasing cholesterol absorption. Male Golden Syrian hamsters were fed for 4 wk diets containing 0.12% cholesterol, and either 4% cellulose or four different viscosity grades of hydroxypropyl methylcellulose (HPMC). Dietary HPMC confers viscosity in the small intestine but is resistant to fermentation. Cholesterol absorption efficiency was measured using the dual isotope ratio method, and plasma and liver cholesterol concentrations were determined enzymatically. Ex vivo viscosity of intestinal contents supernatants was measured using a Wells-Brookfield cone/plate viscometer, and the means of treatment groups ranged from 6 to 6532 mPa · s. Relative to dietary cellulose, all viscosity grades of HPMC resulted in significantly lower cholesterol absorption efficiency, lower plasma cholesterol concentration, and lower liver cholesteryl ester content. The logarithm of intestinal contents ex vivo viscosity was inversely correlated with dietary cholesterol absorption (r2 = 0.84, P = 0.028). Furthermore, dietary cholesterol absorption was positively correlated with plasma cholesterol concentration (r2 = 0.89, P = 0.017) and liver cholesteryl ester content (r2 = 0.96, P = 0.0031). Thus, the data suggest an independent role of intestinal contents viscosity in lowering plasma cholesterol concentration and liver cholesteryl ester content by reducing cholesterol absorption efficiency.

KW - cholesterol absorption

KW - dietary fiber

KW - hamsters

KW - plasma cholesterol

KW - viscosity

UR - http://www.scopus.com/inward/record.url?scp=0029979626&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029979626&partnerID=8YFLogxK

M3 - Article

C2 - 8618144

AN - SCOPUS:0029979626

VL - 126

SP - 1463

EP - 1469

JO - The Journal of nutrition

JF - The Journal of nutrition

SN - 0022-3166

IS - 5

ER -