Incorporating molecular identification of Meloidogyne spp. into a large-scale regional nematode survey

Thomas O Powers, P. G. Mullin, T. S. Harris, L. A. Sutton, R. S. Higgins

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

A regional nematode survey of potato fields was conducted in the central United States during 2002 and 2003. The survey encompassed seven states and included a morphological and molecular examination of nematodes of regulatory concern from 1,929 soil samples. No regulated pest species were recovered during this survey. Meloidogyne juveniles extracted from soil were identified by mitochondrial and 18S ribosomal molecular markers. Eighty-two DNA sequences representing the two marker regions for Meloidogyne species were submitted to GenBank to facilitate evaluation of marker variability. Sufficient 18S variation was observed among some Meloidogyne species to aid in identification; however, nucleotide sequence from this highly conserved region of 18S did not discriminate among M. arenaria, M. incognita, and M. javanica. The mitochondrial gene region provided greater species discrimination and revealed intraspecific variation among many isolates. One nucleotide substitution found in a subset of M. hapla isolates from west Texas and New Mexico affected a DraI restriction site used in the PCR/RFLP diagnostic protocol. None of the mitochondrial sequence variants observed in this study compromised the PCR/RFLP identification protocol for M. chitwoodi. Additional sequence analysis is recommended for validation and evaluation of genetic markers used in diagnostic decisions.

Original languageEnglish (US)
Pages (from-to)226-235
Number of pages10
JournalJournal of Nematology
Volume37
Issue number2
StatePublished - Jun 2005

Fingerprint

Meloidogyne
Nematoda
restriction fragment length polymorphism
Meloidogyne arenaria
nucleotide sequences
genetic markers
Midwestern United States
Meloidogyne incognita
soil sampling
sequence analysis
nucleotides
pests
potatoes
soil
genes

Keywords

  • 18S
  • M. arenaria
  • M. chitwoodi
  • M. incognita
  • M. javanica
  • Meloidogyne spp.
  • Mitochondrial DNA
  • Molecular diagnostics
  • Potato
  • Quarantine nematodes

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Powers, T. O., Mullin, P. G., Harris, T. S., Sutton, L. A., & Higgins, R. S. (2005). Incorporating molecular identification of Meloidogyne spp. into a large-scale regional nematode survey. Journal of Nematology, 37(2), 226-235.

Incorporating molecular identification of Meloidogyne spp. into a large-scale regional nematode survey. / Powers, Thomas O; Mullin, P. G.; Harris, T. S.; Sutton, L. A.; Higgins, R. S.

In: Journal of Nematology, Vol. 37, No. 2, 06.2005, p. 226-235.

Research output: Contribution to journalArticle

Powers, TO, Mullin, PG, Harris, TS, Sutton, LA & Higgins, RS 2005, 'Incorporating molecular identification of Meloidogyne spp. into a large-scale regional nematode survey', Journal of Nematology, vol. 37, no. 2, pp. 226-235.
Powers, Thomas O ; Mullin, P. G. ; Harris, T. S. ; Sutton, L. A. ; Higgins, R. S. / Incorporating molecular identification of Meloidogyne spp. into a large-scale regional nematode survey. In: Journal of Nematology. 2005 ; Vol. 37, No. 2. pp. 226-235.
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