Inactivation of E. Coli affected by medium conductivity in pulsed electric field

Tanya Kirilova Gachovska, Jeyamkondan Subbiah, Harshavardhan Thippareddi, David Marx, Frazer Williams

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

Pulsed electric field PEF is effective in inactivating microorganisms in liquids such as saline water, phosphate buffer, milk and others. PEF treatment efficacy is influenced by number of parameters. Medium conductivity has been a subject of investigation because of the contrary reports. Present study was undertaken to systematic evaluate the effect of medium conductivity on the inactivation of E. coli. The experiments were conducted on E-coli innoculted phosphate buffer (2 to 4.5 mS/cm) in a continuous-flow co-field treatment chamber with an applied electric field strength of 60 kV/cm and 50 Hz pulse frequency. In the first set of experiments, the effect of medium conductivity on E-coli inactivation was evaluated at 49 μs treatment time. In the second set of experiments, the effect of medium conductivity was evaluated at constant specific energies of 54, 77 and 135 J/mL. For the same treatment time, the microbial inactivation was higher in the buffer with higher electivity conductivity. Sub-lethal injury to E. coli. was observed in buffer PEF treatment. Even though the treatment times were different there was no significant effect of on the microbial inactivation to the different conductivity medium when the same specific energy was applied.

Original languageEnglish (US)
Title of host publication2013 19th IEEE Pulsed Power Conference, PPC 2013
DOIs
StatePublished - Dec 4 2013
Event2013 19th IEEE Pulsed Power Conference, PPC 2013 - San Francisco, CA, United States
Duration: Jun 16 2013Jun 21 2013

Publication series

NameDigest of Technical Papers-IEEE International Pulsed Power Conference

Conference

Conference2013 19th IEEE Pulsed Power Conference, PPC 2013
CountryUnited States
CitySan Francisco, CA
Period6/16/136/21/13

Fingerprint

Escherichia coli
Electric fields
Phosphates
Saline water
Experiments
Microorganisms
Liquids

ASJC Scopus subject areas

  • Energy Engineering and Power Technology
  • Electrical and Electronic Engineering

Cite this

Gachovska, T. K., Subbiah, J., Thippareddi, H., Marx, D., & Williams, F. (2013). Inactivation of E. Coli affected by medium conductivity in pulsed electric field. In 2013 19th IEEE Pulsed Power Conference, PPC 2013 [6627703] (Digest of Technical Papers-IEEE International Pulsed Power Conference). https://doi.org/10.1109/PPC.2013.6627703

Inactivation of E. Coli affected by medium conductivity in pulsed electric field. / Gachovska, Tanya Kirilova; Subbiah, Jeyamkondan; Thippareddi, Harshavardhan; Marx, David; Williams, Frazer.

2013 19th IEEE Pulsed Power Conference, PPC 2013. 2013. 6627703 (Digest of Technical Papers-IEEE International Pulsed Power Conference).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Gachovska, TK, Subbiah, J, Thippareddi, H, Marx, D & Williams, F 2013, Inactivation of E. Coli affected by medium conductivity in pulsed electric field. in 2013 19th IEEE Pulsed Power Conference, PPC 2013., 6627703, Digest of Technical Papers-IEEE International Pulsed Power Conference, 2013 19th IEEE Pulsed Power Conference, PPC 2013, San Francisco, CA, United States, 6/16/13. https://doi.org/10.1109/PPC.2013.6627703
Gachovska TK, Subbiah J, Thippareddi H, Marx D, Williams F. Inactivation of E. Coli affected by medium conductivity in pulsed electric field. In 2013 19th IEEE Pulsed Power Conference, PPC 2013. 2013. 6627703. (Digest of Technical Papers-IEEE International Pulsed Power Conference). https://doi.org/10.1109/PPC.2013.6627703
Gachovska, Tanya Kirilova ; Subbiah, Jeyamkondan ; Thippareddi, Harshavardhan ; Marx, David ; Williams, Frazer. / Inactivation of E. Coli affected by medium conductivity in pulsed electric field. 2013 19th IEEE Pulsed Power Conference, PPC 2013. 2013. (Digest of Technical Papers-IEEE International Pulsed Power Conference).
@inproceedings{a2c1d9ab9925416088c6db255a039777,
title = "Inactivation of E. Coli affected by medium conductivity in pulsed electric field",
abstract = "Pulsed electric field PEF is effective in inactivating microorganisms in liquids such as saline water, phosphate buffer, milk and others. PEF treatment efficacy is influenced by number of parameters. Medium conductivity has been a subject of investigation because of the contrary reports. Present study was undertaken to systematic evaluate the effect of medium conductivity on the inactivation of E. coli. The experiments were conducted on E-coli innoculted phosphate buffer (2 to 4.5 mS/cm) in a continuous-flow co-field treatment chamber with an applied electric field strength of 60 kV/cm and 50 Hz pulse frequency. In the first set of experiments, the effect of medium conductivity on E-coli inactivation was evaluated at 49 μs treatment time. In the second set of experiments, the effect of medium conductivity was evaluated at constant specific energies of 54, 77 and 135 J/mL. For the same treatment time, the microbial inactivation was higher in the buffer with higher electivity conductivity. Sub-lethal injury to E. coli. was observed in buffer PEF treatment. Even though the treatment times were different there was no significant effect of on the microbial inactivation to the different conductivity medium when the same specific energy was applied.",
author = "Gachovska, {Tanya Kirilova} and Jeyamkondan Subbiah and Harshavardhan Thippareddi and David Marx and Frazer Williams",
year = "2013",
month = "12",
day = "4",
doi = "10.1109/PPC.2013.6627703",
language = "English (US)",
isbn = "9781467351676",
series = "Digest of Technical Papers-IEEE International Pulsed Power Conference",
booktitle = "2013 19th IEEE Pulsed Power Conference, PPC 2013",

}

TY - GEN

T1 - Inactivation of E. Coli affected by medium conductivity in pulsed electric field

AU - Gachovska, Tanya Kirilova

AU - Subbiah, Jeyamkondan

AU - Thippareddi, Harshavardhan

AU - Marx, David

AU - Williams, Frazer

PY - 2013/12/4

Y1 - 2013/12/4

N2 - Pulsed electric field PEF is effective in inactivating microorganisms in liquids such as saline water, phosphate buffer, milk and others. PEF treatment efficacy is influenced by number of parameters. Medium conductivity has been a subject of investigation because of the contrary reports. Present study was undertaken to systematic evaluate the effect of medium conductivity on the inactivation of E. coli. The experiments were conducted on E-coli innoculted phosphate buffer (2 to 4.5 mS/cm) in a continuous-flow co-field treatment chamber with an applied electric field strength of 60 kV/cm and 50 Hz pulse frequency. In the first set of experiments, the effect of medium conductivity on E-coli inactivation was evaluated at 49 μs treatment time. In the second set of experiments, the effect of medium conductivity was evaluated at constant specific energies of 54, 77 and 135 J/mL. For the same treatment time, the microbial inactivation was higher in the buffer with higher electivity conductivity. Sub-lethal injury to E. coli. was observed in buffer PEF treatment. Even though the treatment times were different there was no significant effect of on the microbial inactivation to the different conductivity medium when the same specific energy was applied.

AB - Pulsed electric field PEF is effective in inactivating microorganisms in liquids such as saline water, phosphate buffer, milk and others. PEF treatment efficacy is influenced by number of parameters. Medium conductivity has been a subject of investigation because of the contrary reports. Present study was undertaken to systematic evaluate the effect of medium conductivity on the inactivation of E. coli. The experiments were conducted on E-coli innoculted phosphate buffer (2 to 4.5 mS/cm) in a continuous-flow co-field treatment chamber with an applied electric field strength of 60 kV/cm and 50 Hz pulse frequency. In the first set of experiments, the effect of medium conductivity on E-coli inactivation was evaluated at 49 μs treatment time. In the second set of experiments, the effect of medium conductivity was evaluated at constant specific energies of 54, 77 and 135 J/mL. For the same treatment time, the microbial inactivation was higher in the buffer with higher electivity conductivity. Sub-lethal injury to E. coli. was observed in buffer PEF treatment. Even though the treatment times were different there was no significant effect of on the microbial inactivation to the different conductivity medium when the same specific energy was applied.

UR - http://www.scopus.com/inward/record.url?scp=84888606302&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84888606302&partnerID=8YFLogxK

U2 - 10.1109/PPC.2013.6627703

DO - 10.1109/PPC.2013.6627703

M3 - Conference contribution

AN - SCOPUS:84888606302

SN - 9781467351676

T3 - Digest of Technical Papers-IEEE International Pulsed Power Conference

BT - 2013 19th IEEE Pulsed Power Conference, PPC 2013

ER -