Inaccurate DNA synthesis in cell extracts of yeast producing active human DNA Polymerase iota

Alena V. Makarova, Corinn Grabow, Leonid V. Gening, Vyacheslav Z. Tarantul, Tahir H Tahirov, Tadayoshi Bessho, Youri I Pavlov

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Mammalian Pol ι has an unusual combination of properties: it is stimulated by Mn2+ ions, can bypass some DNA lesions and misincorporates "G" opposite template "T" more frequently than incorporates the correct "A." We recently proposed a method of detection of Pol ι activity in animal cell extracts, based on primer extension opposite the template T with a high concentration of only two nucleotides, dGTP and dATP (incorporation of "G" versus "A" method of Gening, abbreviated as "misGvA"). We provide unambiguous proof of the "misGvA" approach concept and extend the applicability of the method for the studies of variants of Pol ι in the yeast model system with different cation cofactors. We produced human Pol i in baker's yeast, which do not have a POLI ortholog. The "misGvA" activity is absent in cell extracts containing an empty vector, or producing catalytically dead Pol ι, or Pol ι lacking exon 2, but is robust in the strain producing wild-type Pol ι or its catalytic core, or protein with the active center L62I mutant. The signature pattern of primer extension products resulting from inaccurate DNA synthesis by extracts of cells producing either Pol ι or human Pol η is different. The DNA sequence of the template is critical for the detection of the infidelity of DNA synthesis attributed to DNA Pol ι. The primer/template and composition of the exogenous DNA precursor pool can be adapted to monitor replication fidelity in cell extracts expressing various error-prone Pols or mutator variants of accurate Pols. Finally, we demonstrate that the mutation rates in yeast strains producing human DNA Pols ι and η are not elevated over the control strain, despite highly inaccurate DNA synthesis by their extracts. Copyright:

Original languageEnglish (US)
Article numbere16612
JournalPloS one
Volume6
Issue number1
DOIs
StatePublished - Feb 9 2011

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DNA-directed DNA polymerase
yeast extract
Cell Extracts
Yeast
Yeasts
synthesis
DNA
extracts
cells
Strain control
yeasts
bakers yeast
DNA sequences
Mutation Rate
DNA polymerase iota
lesions (animal)
exons
Saccharomyces cerevisiae
Cations
Exons

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Inaccurate DNA synthesis in cell extracts of yeast producing active human DNA Polymerase iota. / Makarova, Alena V.; Grabow, Corinn; Gening, Leonid V.; Tarantul, Vyacheslav Z.; Tahirov, Tahir H; Bessho, Tadayoshi; Pavlov, Youri I.

In: PloS one, Vol. 6, No. 1, e16612, 09.02.2011.

Research output: Contribution to journalArticle

Makarova, Alena V. ; Grabow, Corinn ; Gening, Leonid V. ; Tarantul, Vyacheslav Z. ; Tahirov, Tahir H ; Bessho, Tadayoshi ; Pavlov, Youri I. / Inaccurate DNA synthesis in cell extracts of yeast producing active human DNA Polymerase iota. In: PloS one. 2011 ; Vol. 6, No. 1.
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