In vivo protective effects of tetrapeptide AcSDKP, with or without granulocyte colony-stimulating factor, on murine progenitor cells after sublethal irradiation

T. Watanabe, G. S. Brown, L. S. Kelsey, Y. Yan, J. D. Jackson, C. Ewel, A. Kessinger, James E Talmadge

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Acetyl-N-Ser-Asp-Lys-Pro (AcSDKP) demonstrated hemato-protective activity in mice after sublethal irradiation (7 Gy). Bone marrow interleukin-3 (IL-3)-responsive colony-forming cells (CFC) and high proliferative potential colony-forming cells (HPP-CFC) were significantly (P < 0.05) increased by day 10 after irradiation in mice receiving a continuous infusion of 1000 ng/day of AcSDKP compared to irradiated control mice. The maximum protective effect for bone marrow progenitors was achieved when AcSDKP was administered for 3 days beginning. 24 hours before irradiation. Other dosages and schedules in relationship to irradiation were less active. Further, when granulocyte colony-stimulating factor (G-CSF) was administered for 10 days after AcSDKP infusion in irradiated mice, significantly increased numbers of IL-3-responsive CFC and HPP-CFC in the bone marrow and spleen were observed 14-21 days after irradiation compared with G-CSF-only control mice. In addition, platelets were significantly (p < 0.05) increased in mice receiving AcSDKP and G-CSF on days 18 and 21 after irradiation compared with mice receiving G-CSF alone. We conclude that AcSDKP has a radioprotective effect in vivo for progenitor cells, and that time of initiation and duration of AcSDKP administration relative to irradiation are crucial for these effects. Further, AcSDKP has a significant additive protective effect not only for progenitor cells but also for platelets when given in combination with G-CSF. We suggest that these in vivo observations provide a basis on which to design optimal clinical hypotheses and protocols.

Original languageEnglish (US)
Pages (from-to)713-721
Number of pages9
JournalExperimental Hematology
Volume24
Issue number6
StatePublished - Jun 6 1996

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Granulocyte Colony-Stimulating Factor
Stem Cells
Interleukin-3
Blood Platelets
Bone Marrow
goralatide
Clinical Protocols
Bone Marrow Cells
Appointments and Schedules
Spleen

Keywords

  • AcSDKP
  • G-CSF
  • Progenitor cells
  • Radioprotective

ASJC Scopus subject areas

  • Molecular Biology
  • Hematology
  • Genetics
  • Cell Biology
  • Cancer Research

Cite this

In vivo protective effects of tetrapeptide AcSDKP, with or without granulocyte colony-stimulating factor, on murine progenitor cells after sublethal irradiation. / Watanabe, T.; Brown, G. S.; Kelsey, L. S.; Yan, Y.; Jackson, J. D.; Ewel, C.; Kessinger, A.; Talmadge, James E.

In: Experimental Hematology, Vol. 24, No. 6, 06.06.1996, p. 713-721.

Research output: Contribution to journalArticle

Watanabe, T. ; Brown, G. S. ; Kelsey, L. S. ; Yan, Y. ; Jackson, J. D. ; Ewel, C. ; Kessinger, A. ; Talmadge, James E. / In vivo protective effects of tetrapeptide AcSDKP, with or without granulocyte colony-stimulating factor, on murine progenitor cells after sublethal irradiation. In: Experimental Hematology. 1996 ; Vol. 24, No. 6. pp. 713-721.
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AU - Kelsey, L. S.

AU - Yan, Y.

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